Click image to see more details
Product Info Summary
| SKU: | A00479-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Leptin Antibody Picoband™
SKU/Catalog Number
A00479-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leptin Antibody Picoband™ catalog # A00479-4. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00479-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Leptin recombinant protein (Position: V22-C167).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00479-4 is reactive to LEP in Mouse, Rat
Applications
A00479-4 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
23 kDa
Calculated molecular weight
18866 MW
Background of Leptin/OB
Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (A00479-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # A00479-4) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 23KD. The expected band size for Leptin is at 18KD.
Click image to see more details
Figure 2. IHC analysis of Leptin using anti-Leptin antibody (A00479-4).
Leptin was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Leptin using anti-Leptin antibody (A00479-4).
Leptin was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Leptin using anti-Leptin antibody (A00479-4).
Leptin was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For LEP (Source: Uniprot.org, NCBI)
Gene Name
LEP
Full Name
Leptin
Weight
18866 MW
Superfamily
leptin family
Alternative Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS LEP LEPD, OB, OBS leptin leptin|leptin (murine obesity homolog)|leptin (obesity homolog, mouse)|obese protein|obese, mouse, homolog of|obesity factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEP, check out the LEP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leptin Antibody Picoband™ (A00479-4)
Hello CJ!
A00479-4 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Stress‐induced cardiac insufficiency relating to abnormal leptin and FKBP12.6 is ameliorated by CPU0213, an endothelin receptor antagonist, which is not affected by the CYP3A suppressing effect of erythromycin
Trigonelline Inhibits Inflammation and Protects β Cells to Prevent Fetal Growth Restriction during Pregnancy in a Mouse Model of Diabetes
Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid
Immunohistochemical Localization of Leptin and Leptin-Receptor Proteins in Different Tissues of Chinese Alligator, Alligator sinensis. HuaBin Zhang,YaSen Li,Tao Pan,Peng Yan,En Li,Hui Xue,XiaoBing Wu
Species: Chinese Alligator
A00479-4 usage in article: APP:IHC, SAMPLE:TESTIS,SUPERMADUCT,EPIDIDYMIS, OVARY,OVIDUCT,STOMACH,INTESTINE AND ADIPOSE TISSUE, DILUTION:1:100
lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Leptin Antibody Picoband™
Question
Is a blocking peptide available for product anti-Leptin antibody (A00479-4)?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We do provide the blocking peptide for product anti-Leptin antibody (A00479-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-30
Question
I am interested in using your anti-Leptin antibody for cellular response to retinoic acid studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.
W. Miller
Verified customer
Asked: 2020-04-09
Answer
We appreciate your inquiry. This A00479-4 anti-Leptin antibody is validated on mouse lung tissue, small intestine tissue, kidney tissue, rat lung tissue. It is guaranteed to work for ELISA, IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-09
Question
We have been able to see staining in mouse placenta. Any tips? Is anti-Leptin antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
Based on literature placenta does express LEP. Based on Uniprot.org, LEP is expressed in adipose tissue of abdominal region, placenta, among other tissues. Regarding which tissues have LEP expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-26
Question
Can you help my question with product A00479-4, anti-Leptin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00479-4 anti-Leptin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-25
Question
We were content with the WB result of your anti-Leptin antibody. However we have observed positive staining in placenta secreted. using this antibody. Is that expected? Could you tell me where is LEP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
From literature, placenta does express LEP. Generally LEP expresses in secreted. Regarding which tissues have LEP expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-31
Question
I see that the anti-Leptin antibody A00479-4 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-17
Question
We purchased anti-Leptin antibody for WB on adipose tissue of abdominal region a few years ago. I am using rat, and We are going to use the antibody for ELISA next. I am interested in examining adipose tissue of abdominal region as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
I looked at the website and datasheets of our anti-Leptin antibody and it seems that A00479-4 has been validated on rat in both WB and ELISA. Thus A00479-4 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Leptin antibody A00479-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-04
Question
We are currently using anti-Leptin antibody A00479-4 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
The anti-Leptin antibody (A00479-4) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-06
Question
Is this A00479-4 anti-Leptin antibody reactive to the isotypes of LEP?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
The immunogen of A00479-4 anti-Leptin antibody is E. coli-derived mouse Leptin recombinant protein (Position: V22-C167). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-09
Question
Will anti-Leptin antibody A00479-4 work for ELISA with adipose tissue of abdominal region?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
According to the expression profile of adipose tissue of abdominal region, LEP is highly expressed in adipose tissue of abdominal region. So, it is likely that anti-Leptin antibody A00479-4 will work for ELISA with adipose tissue of abdominal region.
Boster Scientific Support
Answered: 2018-06-13
Question
Does A00479-4 anti-Leptin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
As indicated on the product datasheet, A00479-4 anti-Leptin antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-09
Question
See below the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Leptin antibody A00479-4. Please let me know if you require anything else.
N. Thomas
Verified customer
Asked: 2017-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-21
Question
We want to test anti-Leptin antibody A00479-4 on mouse adipose tissue of abdominal region for research purposes, then I may be interested in using anti-Leptin antibody A00479-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Anderson
Verified customer
Asked: 2015-06-23
Answer
The products we sell, including anti-Leptin antibody A00479-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-23
Question
Is there a BSA free version of anti-Leptin antibody A00479-4 available?
W. Wu
Verified customer
Asked: 2015-05-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Leptin antibody A00479-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-19
Question
I was wanting to use your anti-Leptin antibody for ELISA for mouse adipose tissue of abdominal region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adipose tissue of abdominal region identification?
M. Edwards
Verified customer
Asked: 2014-04-01
Answer
It shows on the product datasheet, A00479-4 anti-Leptin antibody has been validated for ELISA, IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adipose tissue of abdominal region in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-01
