Click image to see more details
Product Info Summary
| SKU: | A00479-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Leptin Antibody Picoband®
SKU/Catalog Number
A00479-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leptin Antibody Picoband® catalog # A00479-2. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00479-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Leptin recombinant protein (Position: V22-C167). Rat Leptin shares 83.6% and 95.9% amino acid (aa) sequence identity with human and mouse Leptin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00479-2 is reactive to Lep in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
18866 MW
Background of Leptin/OB
Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00479-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml,
Western blot, 0.1-0.5μg/ml, Rat,
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (A00479-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat Leptin protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # A00479-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 16KD. The expected band size for Leptin is at 16KD.
Protein Target Info & Infographic
Gene/Protein Information For Lep (Source: Uniprot.org, NCBI)
Gene Name
Lep
Full Name
Leptin
Weight
18866 MW
Superfamily
leptin family
Alternative Names
Leptin;Obesity factor;Lep;Ob; LEP LEPD, OB, OBS leptin leptin|leptin (murine obesity homolog)|leptin (obesity homolog, mouse)|obese protein|obese, mouse, homolog of|obesity factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lep, check out the Lep Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lep: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leptin Antibody Picoband® (A00479-2)
Hello CJ!
A00479-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Leptin Antibody Picoband®
Question
We are currently using anti-Leptin antibody A00479-2 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The anti-Leptin antibody (A00479-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-16
Question
We ordered your anti-Leptin antibody for ELISA on placenta in the past. I am using rat, and I plan to use the antibody for WB next. I am looking for examining placenta as well as adipose tissue of abdominal region in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
I took a look at the website and datasheets of our anti-Leptin antibody and it appears that A00479-2 has been validated on rat in both ELISA and WB. Thus A00479-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-04
Question
We have observed staining in rat placenta. What should we do? Is anti-Leptin antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
From what I have seen in literature placenta does express LEP. From what I have seen in Uniprot.org, LEP is expressed in adipose tissue of abdominal region, placenta, among other tissues. Regarding which tissues have LEP expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-19
Question
I have attached the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Leptin antibody A00479-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
My boss were well pleased with the WB result of your anti-Leptin antibody. However we have seen positive staining in placenta secreted. using this antibody. Is that expected? Could you tell me where is LEP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
From literature, placenta does express LEP. Generally LEP expresses in secreted. Regarding which tissues have LEP expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-01
Question
My question regards to test anti-Leptin antibody A00479-2 on rat adipose tissue of abdominal region for research purposes, then I may be interested in using anti-Leptin antibody A00479-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Singh
Verified customer
Asked: 2019-07-31
Answer
The products we sell, including anti-Leptin antibody A00479-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-31
Question
Is there a BSA free version of anti-Leptin antibody A00479-2 available?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Leptin antibody A00479-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-26
Question
I see that the anti-Leptin antibody A00479-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-03
Question
I was wanting to use your anti-Leptin antibody for WB for rat adipose tissue of abdominal region on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adipose tissue of abdominal region identification?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
It shows on the product datasheet, A00479-2 anti-Leptin antibody has been tested for ELISA, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adipose tissue of abdominal region in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
Would anti-Leptin antibody A00479-2 work for WB with adipose tissue of abdominal region?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
According to the expression profile of adipose tissue of abdominal region, LEP is highly expressed in adipose tissue of abdominal region. So, it is likely that anti-Leptin antibody A00479-2 will work for WB with adipose tissue of abdominal region.
Boster Scientific Support
Answered: 2018-10-26
Question
Can you help my question with product A00479-2, anti-Leptin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Gonzalez
Verified customer
Asked: 2017-11-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00479-2 anti-Leptin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-10
Question
Is this A00479-2 anti-Leptin antibody reactive to the isotypes of LEP?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
The immunogen of A00479-2 anti-Leptin antibody is E. coli-derived rat Leptin recombinant protein (Position: V22-C167). Rat Leptin shares 83.6% and 95.9% amino acid (aa) sequence identity with human and mouse Leptin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-11
Question
Is a blocking peptide available for product anti-Leptin antibody (A00479-2)?
C. Bhatt
Verified customer
Asked: 2016-01-29
Answer
We do provide the blocking peptide for product anti-Leptin antibody (A00479-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-29
Question
Does A00479-2 anti-Leptin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Patel
Verified customer
Asked: 2016-01-05
Answer
You can see on the product datasheet, A00479-2 anti-Leptin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-01-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Leptin antibody A00479-2. Let me know if you need anything else.
T. Roberts
Verified customer
Asked: 2014-08-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-07
