Product Info Summary
SKU: | A00751T322 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-Phospho-DDX3 (T322) DDX3X Antibody
SKU/Catalog Number
A00751T322
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-DDX3 (T322) DDX3X Antibody catalog # A00751T322. Tested in ELISA, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-DDX3 (T322) DDX3X Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00751T322)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human DDX3 around the phosphorylation site of T322.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00751T322 is reactive to DDX3X in Human, Mouse, Rat
Applications
A00751T322 is guaranteed for ELISA, IF, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
73243 MW
Background of DDX3
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Ana V. Miletic, J. Biol. Chem., Dec 2006; 281: 38257 - 38265.
Christopher C. Stebbins, Mol. Cell. Biol., Sep 2003; 23: 6291 - 6299.
Vered Schapira, Cancer Res., Jun 2006; 66: 6183 - 6191.
Maya Groysman, J. Biol. Chem., Dec 2002; 277: 50121 - 50130.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of DDX3X using Anti-Phospho-DDX3 (T322) DDX3X Antibody (A00751T322).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 2. Immunohistochemistry validation of DDX3X using Anti-Phospho-DDX3 (T322) DDX3X Antibody (A00751T322).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CProtein Target Info & Infographic
Gene/Protein Information For DDX3X (Source: Uniprot.org, NCBI)
Gene Name
DDX3X
Full Name
ATP-dependent RNA helicase DDX3X
Weight
73243 MW
Superfamily
DEAD box helicase family
Alternative Names
DBXCAP-Rf; DDX14; DDX3DEAD/H box-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; EC 3.6.1; EC 3.6.4.13; helicase like protein 2; Helicase-like protein 2; HLP2ATP-dependent RNA helicase DDX3X DDX3X CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB DEAD-box helicase 3 X-linked ATP-dependent RNA helicase DDX3X|DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked|DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|DEAD box protein 3, X-chromosomal|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX3X, check out the DDX3X Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX3X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-DDX3 (T322) DDX3X Antibody (A00751T322)
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