Product Info Summary
SKU: | M00751 |
---|---|
Size: | 100 µg |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2)
SKU/Catalog Number
M00751
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2) (Catalog# M00751). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00751)
Host
Mouse
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Monoclonal
Clone Number
ABM27H2
Isotype
IgG2b Kappa
Immunogen
A partial length recombinant DDX3 protein (amino acids 10-230)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00751 is reactive to DDX3X in Human, Mouse
Reconstitution
Reconstitute with distilled water.
Calculated molecular weight
73243 MW
Background of DDX3
This monoclonal antibody is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00751 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of DDX3. Anti-DDX3 antibody (Clone: ABM27H2) was used at 2 μg/ml on (1) MCF-7,(2) NIH3T3 (3) HEK293 and (4) EL-4 lysates.
Protein Target Info & Infographic
Gene/Protein Information For DDX3X (Source: Uniprot.org, NCBI)
Gene Name
DDX3X
Full Name
ATP-dependent RNA helicase DDX3X
Weight
73243 MW
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX3X;3.6.4.13;DEAD box protein 3, X-chromosomal;DEAD box, X isoform;Helicase-like protein 2;HLP2;DDX3X;DBX, DDX3; DDX3X CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB DEAD-box helicase 3 X-linked ATP-dependent RNA helicase DDX3X|DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked|DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|DEAD box protein 3, X-chromosomal|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX3X, check out the DDX3X Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX3X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2) (M00751)
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No publications found for M00751
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2)
Question
Will anti-DDX3 antibody (Monoclonal, ABM27H2) M00751 work for WB with hippocampus liver?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
According to the expression profile of hippocampus liver, DDX3X is highly expressed in hippocampus liver. So, it is likely that anti-DDX3 antibody (Monoclonal, ABM27H2) M00751 will work for WB with hippocampus liver.
Boster Scientific Support
Answered: 2020-05-07
Question
Is a blocking peptide available for product anti-DDX3 antibody (Monoclonal, ABM27H2) (M00751)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do provide the blocking peptide for product anti-DDX3 antibody (Monoclonal, ABM27H2) (M00751). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-27
Question
Here is the WB image, lot number and protocol we used for hippocampus liver using anti-DDX3 antibody (Monoclonal, ABM27H2) M00751. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-25
Question
I was wanting to use your anti-DDX3 antibody (Monoclonal, ABM27H2) for WB for human hippocampus liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus liver identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
You can see on the product datasheet, M00751 anti-DDX3 antibody (Monoclonal, ABM27H2) has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Our lab want to know about to test anti-DDX3 antibody (Monoclonal, ABM27H2) M00751 on human hippocampus liver for research purposes, then I may be interested in using anti-DDX3 antibody (Monoclonal, ABM27H2) M00751 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The products we sell, including anti-DDX3 antibody (Monoclonal, ABM27H2) M00751, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-01
Question
I see that the anti-DDX3 antibody (Monoclonal, ABM27H2) M00751 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-26