Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2)

Product Name

Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2)

View all DDX3 Antibodies

SKU/Catalog Number

M00751

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2) (Catalog# M00751). Tested in WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-DDX3 DDX3X Antibody (Monoclonal, ABM27H2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00751)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

ABM27H2

Isotype

IgG2b Kappa

Immunogen

A partial length recombinant DDX3 protein (amino acids 10-230)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00751 is reactive to DDX3X in Human, Mouse

Applications

M00751 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

73243 MW

Background of DDX3

This monoclonal antibody is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml

Validation Images & Assay Conditions

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Western blot analysis of DDX3. Anti-DDX3 antibody (Clone: ABM27H2) was used at 2 μg/ml on (1) MCF-7,(2) NIH3T3 (3) HEK293 and (4) EL-4 lysates.

Gene/Protein Information For DDX3X (Source: Uniprot.org, NCBI)

Alternative Names

DBXCAP-Rf; DDX14; DDX3DEAD/H box-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; EC 3.6.1; EC 3.6.4.13; helicase like protein 2; Helicase-like protein 2; HLP2ATP-dependent RNA helicase DDX3X DDX3X CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB DEAD-box helicase 3 X-linked ATP-dependent RNA helicase DDX3X|DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked|DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|DEAD box protein 3, X-chromosomal|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase-like protein 2

For more info on DDX3X, check out the DDX3X Infographic

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In this infographic, you will see the following information for DDX3X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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