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Product Info Summary
| SKU: | P00905 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-DDR1 (Y513) Antibody
SKU/Catalog Number
P00905
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-DDR1 (Y513) Antibody catalog # P00905. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-DDR1 (Y513) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00905)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DDR1 around the phosphorylation site of Tyr513. AA range:479-528
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00905 is reactive to DDR1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
101128 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00905 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-DDR1 (Y513) polyclonal antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DDR1 (Phospho-Tyr513) antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using DDR1 (Phospho-Tyr513) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using DDR1 (Phospho-Tyr513) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For DDR1 (Source: Uniprot.org, NCBI)
Gene Name
DDR1
Full Name
Epithelial discoidin domain-containing receptor 1
Weight
101128 MW
Superfamily
protein kinase superfamily
Alternative Names
Epithelial discoidin domain-containing receptor 1;Epithelial discoidin domain receptor 1;2.7.10.1;CD167 antigen-like family member A;Cell adhesion kinase;Discoidin receptor tyrosine kinase;HGK2;Mammary carcinoma kinase 10;MCK-10;Protein-tyrosine kinase 3A;Protein-tyrosine kinase RTK-6;TRK E;Tyrosine kinase DDR;Tyrosine-protein kinase CAK;CD167a;DDR1;CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE; DDR1 CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE discoidin domain receptor tyrosine kinase 1 epithelial discoidin domain-containing receptor 1|CD167 -like family member A|PTK3A protein tyrosine kinase 3A|cell adhesion kinase|mammary carcinoma kinase 10|neuroepithelial tyrosine kinase|neurotrophic tyrosine kinase, receptor, type 4|protein-tyrosine kinase RTK-6|tyrosine kinase DDR|tyrosine-protein kinase CAK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDR1, check out the DDR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-DDR1 (Y513) Antibody (P00905)
Hello CJ!
P00905 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Complement C1q stimulates the progression of hepatocellular tumor through the activation of discoidin domain receptor 1
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-DDR1 (Y513) Antibody
Question
Will anti-Phospho-DDR1 (Y513) antibody P00905 work on horse WB with lung?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Our lab technicians have not tested anti-Phospho-DDR1 (Y513) antibody P00905 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Phospho-DDR1 (Y513) antibody P00905 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
See attached the WB image, lot number and protocol we used for mammary carcinoma using anti-Phospho-DDR1 (Y513) antibody P00905. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma using anti-Phospho-DDR1 (Y513) antibody P00905. Let me know if you need anything else.
S. Huang
Verified customer
Asked: 2019-12-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-25
Question
We have observed staining in rat peripheral blood leukocyte. Do you have any suggestions? Is anti-Phospho-DDR1 (Y513) antibody supposed to stain peripheral blood leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
According to literature peripheral blood leukocyte does express DDR1. According to Uniprot.org, DDR1 is expressed in corpus callosum, brain keratinocyte, placenta, ovary, fetal liver, lung, brain placenta, brain, peripheral blood leukocyte, brain muscle, mammary carcinoma, melanocyte, among other tissues. Regarding which tissues have DDR1 expression, here are a few articles citing expression in various tissues:
Brain, and Keratinocyte, Pubmed ID: 8226977
Brain, and Muscle, Pubmed ID: 15489334
Fetal liver, Pubmed ID: 8302582
Mammary carcinoma, Pubmed ID: 7845687, 7834423
Melanocyte, Pubmed ID: 8247543, 9659899
Ovary, Pubmed ID: 7848919
Peripheral blood leukocyte, Pubmed ID: 16702430
Placenta, Pubmed ID: 8390675
Boster Scientific Support
Answered: 2019-11-20
Question
Does P00905 anti-Phospho-DDR1 (Y513) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
As indicated on the product datasheet, P00905 anti-Phospho-DDR1 (Y513) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-Phospho-DDR1 (Y513) antibody P00905 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-Phospho-DDR1 (Y513) antibody (P00905) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
Can you help my question with product P00905, anti-Phospho-DDR1 (Y513) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00905 anti-Phospho-DDR1 (Y513) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
I was wanting to use your anti-Phospho-DDR1 (Y513) antibody for WB for human mammary carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
It shows on the product datasheet, P00905 anti-Phospho-DDR1 (Y513) antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mammary carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-10
Question
Do you have a BSA free version of anti-Phospho-DDR1 (Y513) antibody P00905 available?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-DDR1 (Y513) antibody P00905 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-26
Question
We were satisfied with the WB result of your anti-Phospho-DDR1 (Y513) antibody. However we have seen positive staining in brain placenta isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is DDR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-30
Answer
Based on literature, brain placenta does express DDR1. Generally DDR1 expresses in isoform 1: cell membrane. Regarding which tissues have DDR1 expression, here are a few articles citing expression in various tissues:
Brain, and Keratinocyte, Pubmed ID: 8226977
Brain, and Muscle, Pubmed ID: 15489334
Fetal liver, Pubmed ID: 8302582
Mammary carcinoma, Pubmed ID: 7845687, 7834423
Melanocyte, Pubmed ID: 8247543, 9659899
Ovary, Pubmed ID: 7848919
Peripheral blood leukocyte, Pubmed ID: 16702430
Placenta, Pubmed ID: 8390675
Boster Scientific Support
Answered: 2019-04-30
Question
I see that the anti-Phospho-DDR1 (Y513) antibody P00905 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-15
Question
Is a blocking peptide available for product anti-Phospho-DDR1 (Y513) antibody (P00905)?
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
We do provide the blocking peptide for product anti-Phospho-DDR1 (Y513) antibody (P00905). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-11
Question
Would anti-Phospho-DDR1 (Y513) antibody P00905 work for WB with mammary carcinoma?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
According to the expression profile of mammary carcinoma, DDR1 is highly expressed in mammary carcinoma. So, it is likely that anti-Phospho-DDR1 (Y513) antibody P00905 will work for WB with mammary carcinoma.
Boster Scientific Support
Answered: 2018-04-30
Question
My question regards to test anti-Phospho-DDR1 (Y513) antibody P00905 on human mammary carcinoma for research purposes, then I may be interested in using anti-Phospho-DDR1 (Y513) antibody P00905 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The products we sell, including anti-Phospho-DDR1 (Y513) antibody P00905, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-19
Question
Is this P00905 anti-Phospho-DDR1 (Y513) antibody reactive to the isotypes of DDR1?
A. Zhang
Verified customer
Asked: 2014-07-04
Answer
The immunogen of P00905 anti-Phospho-DDR1 (Y513) antibody is Synthesized peptide derived from human DDR1 around the phosphorylation site of Y513. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-04
