Product Info Summary
SKU: | PA1429 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-MIG/CXCL9 Antibody Picoband®
SKU/Catalog Number
PA1429
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIG/CXCL9 Antibody catalog # PA1429. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIG/CXCL9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1429)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCL9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1429 is reactive to CXCL9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
14019 MW
Background of CXCL9/MIG
MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines. This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1429 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-CXCL9 antibody, PA1429, Western blotting
Lane 1: Recombinant Human CXCL9 Protein 10ng
Lane 2: Recombinant Human CXCL9 Protein 5ng
Lane 3: Recombinant Human CXCL9 Protein 2.5ng
Click image to see more details
Anti-CXCL9 antibody, PA1429, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CXCL9 (Source: Uniprot.org, NCBI)
Gene Name
CXCL9
Full Name
C-X-C motif chemokine 9
Weight
14019 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 9;Gamma-interferon-induced monokine;Monokine induced by interferon-gamma;HuMIG;MIG;Small-inducible cytokine B9;CXCL9;CMK, MIG, SCYB9; CXCL9 CMK, Humig, MIG, SCYB9, crg-10 C-X-C motif chemokine ligand 9 C-X-C motif chemokine 9|chemokine (C-X-C motif) ligand 9|gamma-interferon-induced monokine|monokine induced by gamma interferon|monokine induced by interferon-gamma|small-inducible cytokine B9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL9, check out the CXCL9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MIG/CXCL9 Antibody Picoband® (PA1429)
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No publications found for PA1429
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Customer Q&As
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6 Customer Q&As for Anti-MIG/CXCL9 Antibody Picoband®
Question
I see that the anti-MIG/CXCL9 antibody PA1429 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-21
Question
Can you help my question with product PA1429, anti-MIG/CXCL9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1429 anti-MIG/CXCL9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-01
Question
Is this PA1429 anti-MIG/CXCL9 antibody reactive to the isotypes of CXCL9?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The immunogen of PA1429 anti-MIG/CXCL9 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CXCL9 (37-51aa NQGTIHLQSLKDLKQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-MIG/CXCL9 antibody PA1429. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-07
Question
See attached the WB image, lot number and protocol we used for lung using anti-MIG/CXCL9 antibody PA1429. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
I was wanting to use your anti-MIG/CXCL9 antibody for IHC for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
You can see on the product datasheet, PA1429 anti-MIG/CXCL9 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-22