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Product Info Summary
| SKU: | EK0733 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
Product info
Product Name
Mouse CXCL9/Mig ELISA Kit PicoKine®
SKU/Catalog Number
EK0733
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CXCL9/Mig ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl9 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CXCL9/Mig ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0733)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: T22-T126
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0733 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0733 is reactive to Cxcl9 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0733 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL9/MIG
Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0733-CAP | Anti-Mouse Cxcl9 Pre-coated 96-well strip microplate | 1 |
| EK0733-ST | Mouse Cxcl9 Standard | 2 vials, 10 ng/tube |
| EK0733-DA | Mouse Cxcl9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.012 | 0.031 | 0.074 | 0.157 | 0.305 | 0.587 | 1.032 | 1.901 |
Data Example Images
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Mouse CXCL9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL9/MIG, Dilution ratio of 1:1, concentration in serum and plasma is 0-710g/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL9/Mig ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 95 | 297 | 1865 | 96 | 268 | 1558 |
| Standard deviation | 3.89 | 9.92 | 98.66 | 4.86 | 9.11 | 36.77 |
| CV(%) | 4.1% | 3.3% | 5.3% | 5.1% | 3.4% | 2.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL9/MIG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 86 | 84 | 92 | 82 | 86 | 3.74 | 4.3% |
| Sample 2 | 228 | 207 | 217 | 212 | 216 | 7.77 | 3.5% |
| Sample 3 | 891 | 891 | 830 | 879 | 872 | 25.16 | 2.8% |
Protein Target Info & Infographic
Gene/Protein Information For Cxcl9 (Source: Uniprot.Org, NCBI)
Gene Name
Cxcl9
Full Name
C-X-C motif chemokine 9
Weight
14444 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 9;Gamma-interferon-induced monokine;Monokine induced by interferon-gamma;MIG;MuMIG;Protein m119;Small-inducible cytokine B9;Cxcl9;Mig, Scyb9; CXCL9 CMK, Humig, MIG, SCYB9, crg-10 C-X-C motif chemokine ligand 9 C-X-C motif chemokine 9|chemokine (C-X-C motif) ligand 9|gamma-interferon-induced monokine|monokine induced by gamma interferon|monokine induced by interferon-gamma|small-inducible cytokine B9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cxcl9, check out the Cxcl9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CXCL9/Mig ELISA Kit PicoKine® (EK0733)
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Customer Q&As
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6 Customer Q&As for Mouse CXCL9/Mig ELISA Kit PicoKine®
Question
Does EK0733 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse CXCL9/Mig ELISA Kit PicoKine® (EK0733) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how to proceed with the analysis of ELISA data? I have performed CXCL9 level in serum.
Verified Customer
Verified customer
Asked: 2020-09-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-20
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-10-09
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
M. Jackson
Verified customer
Asked: 2019-05-02
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-02
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CXCL9 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-12-01
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL9 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-01
Question
Q: is it okay to use edta plasma as samples in Mouse CXCL9 Picokine® ELISA Kit (Catalog # EK0733)?
Verified Customer
Verified customer
Asked: 2018-05-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CXCL9 causing disruption of its protein structure. CXCL9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL9 ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-12
