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Product Info Summary
| SKU: | P00543 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00543
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody catalog # P00543. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00543)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GHC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin E1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00543 is reactive to CCNE1 in Human
Reconstitution
Observed Molecular Weight
185 kDa
Calculated molecular weight
47077 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00543 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: JAR cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Cyclin E1 (T77) expression in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CCNE1 (Source: Uniprot.org, NCBI)
Gene Name
CCNE1
Full Name
G1/S-specific cyclin-E1
Weight
47077 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-E1;CCNE1;CCNE; CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNE1, check out the CCNE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody (P00543)
Hello CJ!
P00543 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu Y, Tao Y, Chen Y, Xu W. Plos One. 2012;7(11):E48917. Doi: 10.1371/Journal.Pone.0048917. Epub 2012 Nov 7. Rhoc Regulates The Proliferation Of Gastric Cancer Cells Through Interaction With Iqgap1.
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-Cyclin E1 (T77) Rabbit Monoclonal antibody P00543 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The anti-Phospho-Cyclin E1 (T77) Rabbit Monoclonal antibody (P00543) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-18
