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Product Info Summary
| SKU: | M00166 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00166
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK2 Rabbit Monoclonal Antibody catalog # M00166. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00166)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cdk2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00166 is reactive to CDK2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
33930 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00166 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: human Jurkat whole cell, human HepG2 whole cell, human U2OS whole cell, human K562 whole cell, rat L6 whole cell, rat C6 whole cell, mouse C2C12 whole cell, mouse NIH/3T3 whole cell
IHC: human beast carcinoma tissue, Rat heart tissue, Rat pancreas tissue, Human non-Hodgkin's lymphoma tissue, Human liver cancer tissue, Mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDK2 using anti-CDK2 antibody (M00166).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat L6 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse C2C12 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK2 antigen affinity purified monoclonal antibody (Catalog # M00166) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK2 at approximately 30 kDa. The expected band size for CDK2 is at 34 kDa.
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Immunohistochemical analysis of paraffin-embedded human beast carcinoma, using CDK2 Antibody.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse testis, using the Antibody at 1:100 dilution.
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Immunofluorescent analysis of Hela cells, using CDK2 Antibody .
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDK2 (Source: Uniprot.org, NCBI)
Gene Name
CDK2
Full Name
Cyclin-dependent kinase 2
Weight
33930 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 2;2.7.11.22;Cell division protein kinase 2;p33 protein kinase;CDK2;CDKN2; CDK2 CDKN2, p33(CDK2) cyclin dependent kinase 2 cyclin-dependent kinase 2|cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK2, check out the CDK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK2 Rabbit Monoclonal Antibody (M00166)
Hello CJ!
M00166 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IL-2R%u03B1 up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell %u2026
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 %u2026
Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Liu X, Lin X, Mi Y, Li J, Zhang C. Oxid Med Cell Longev. 2018 Jan 9;2018:9390810. doi: 10.1155/2018/9390810. eCollection 2018. Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CDK2 Rabbit Monoclonal Antibody
Question
I have a question about product M00166, anti-CDK2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00166 anti-CDK2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
Our team were content with the WB result of your anti-CDK2 Rabbit Monoclonal antibody. However we have seen positive staining in placenta cytoskeleton using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?
S. Anderson
Verified customer
Asked: 2019-12-18
Answer
Based on literature, placenta does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-CDK2 Rabbit Monoclonal antibody M00166 for rat tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The anti-CDK2 Rabbit Monoclonal antibody (M00166) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-11
Question
Will M00166 anti-CDK2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
It shows on the product datasheet, M00166 anti-CDK2 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-12
Question
Is a blocking peptide available for product anti-CDK2 Rabbit Monoclonal antibody (M00166)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We do provide the blocking peptide for product anti-CDK2 Rabbit Monoclonal antibody (M00166). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-14
Question
Is this M00166 anti-CDK2 Rabbit Monoclonal antibody reactive to the isotypes of CDK2?
K. Parker
Verified customer
Asked: 2019-02-18
Answer
The immunogen of M00166 anti-CDK2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cdk2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-18
Question
Do you have a BSA free version of anti-CDK2 Rabbit Monoclonal antibody M00166 available?
E. Bhatt
Verified customer
Asked: 2018-12-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDK2 Rabbit Monoclonal antibody M00166 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-07
Question
I was wanting to use your anti-CDK2 Rabbit Monoclonal antibody for IHC for human left lobe of thyroid gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human left lobe of thyroid gland identification?
L. Anderson
Verified customer
Asked: 2018-11-19
Answer
It shows on the product datasheet, M00166 anti-CDK2 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human left lobe of thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-19
Question
We want to test anti-CDK2 Rabbit Monoclonal antibody M00166 on human left lobe of thyroid gland for research purposes, then I may be interested in using anti-CDK2 Rabbit Monoclonal antibody M00166 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
The products we sell, including anti-CDK2 Rabbit Monoclonal antibody M00166, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-18
Question
I see that the anti-CDK2 Rabbit Monoclonal antibody M00166 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-27
Question
We bought anti-CDK2 Rabbit Monoclonal antibody for WB on leukemic t-cell a few years ago. I am using mouse, and We are going to use the antibody for IP next. My lab would like examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
I viewed the website and datasheets of our anti-CDK2 Rabbit Monoclonal antibody and it seems that M00166 has been validated on mouse in both WB and IP. Thus M00166 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-17
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-CDK2 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
W. Krishna
Verified customer
Asked: 2017-06-27
Answer
According to literature cervix carcinoma does express CDK2. According to Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-06-27
Question
See below the WB image, lot number and protocol we used for left lobe of thyroid gland using anti-CDK2 Rabbit Monoclonal antibody M00166. Please let me know if you require anything else.
E. Jackson
Verified customer
Asked: 2016-04-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-04
Question
Will anti-CDK2 Rabbit Monoclonal antibody M00166 work on zebrafish IHC with leukemic t-cell?
K. Li
Verified customer
Asked: 2016-03-01
Answer
Our lab technicians have not tested anti-CDK2 Rabbit Monoclonal antibody M00166 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CDK2 Rabbit Monoclonal antibody M00166 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish leukemic t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left lobe of thyroid gland using anti-CDK2 Rabbit Monoclonal antibody M00166. Let me know if you need anything else.
N. Johnson
Verified customer
Asked: 2015-07-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-16
Question
Would anti-CDK2 Rabbit Monoclonal antibody M00166 work for IHC with left lobe of thyroid gland?
J. Williams
Verified customer
Asked: 2015-05-06
Answer
According to the expression profile of left lobe of thyroid gland, CDK2 is highly expressed in left lobe of thyroid gland. So, it is likely that anti-CDK2 Rabbit Monoclonal antibody M00166 will work for IHC with left lobe of thyroid gland.
Boster Scientific Support
Answered: 2015-05-06
Question
I was wanting to use using your anti-CDK2 Rabbit Monoclonal antibody for scf(skp2)-mediated degradation of p27/p21 studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
H. Banerjee
Verified customer
Asked: 2013-09-03
Answer
I appreciate your inquiry. This M00166 anti-CDK2 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-09-03
