Click image to see more details
Product Info Summary
| SKU: | PA2125-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cyclin-dependent kinase 6 Cdk6 Antibody Picoband®
SKU/Catalog Number
PA2125-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin-dependent kinase 6 Cdk6 Antibody catalog # PA2125-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin-dependent kinase 6 Cdk6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2125-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk6, identical to the related rat sequence, and different from the related human sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2125-2 is reactive to Cdk6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
37028 MW
Background of CDK6
Cell division protein kinase 6, also called Plstire, is an enzyme that in humans is encoded by the CDK6 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. Radiation hybrid analysis and inclusion within a mapped clone place the CDK6 gene at 7q21. Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation and promotes G1/S transition. This gene also involved in initiation and maintenance of cell cycle exit during cell differentiation. It prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types. CDK6 also plays a role in promoting the proliferation of beta-cells in pancreatic islets of Langerhans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2125-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, Rat Lung Tissue,
Validation Images & Assay Conditions
Click image to see more details
Anti-Cdk6 antibody, PA2125-2, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Protein Target Info & Infographic
Gene/Protein Information For Cdk6 (Source: Uniprot.org, NCBI)
Gene Name
Cdk6
Full Name
Cyclin-dependent kinase 6
Weight
37028 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 6;2.7.11.22;CR2 protein kinase;CRK2;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE;Cdk6;Cdkn6, Crk2; CDK6 MCPH12, PLSTIRE cyclin dependent kinase 6 cyclin-dependent kinase 6|cell division protein kinase 6|serine/threonine-protein kinase PLSTIRE
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cdk6, check out the Cdk6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdk6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin-dependent kinase 6 Cdk6 Antibody Picoband® (PA2125-2)
Hello CJ!
PA2125-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway
Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.
Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Cyclin-dependent kinase 6 Cdk6 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal tissue using anti-Cdk6 antibody PA2125-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
I was wanting to use using your anti-Cdk6 antibody for hemopoiesis studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
T. Yang
Verified customer
Asked: 2020-03-17
Answer
We appreciate your inquiry. This PA2125-2 anti-Cdk6 antibody is validated on rat lung tissue, testis tissue, tissue lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-17
Question
I was wanting to use your anti-Cdk6 antibody for WB for mouse adrenal tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adrenal tissue identification?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
You can see on the product datasheet, PA2125-2 anti-Cdk6 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adrenal tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
Is this PA2125-2 anti-Cdk6 antibody reactive to the isotypes of CDK6?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The immunogen of PA2125-2 anti-Cdk6 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk6(291-310aa AYGALNHPYFQDLERYKDNL), identical to the related rat sequence, and different from the related human sequence by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-10
Question
Would anti-Cdk6 antibody PA2125-2 work on zebrafish WB with adrenal tissue?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Our lab technicians have not tested anti-Cdk6 antibody PA2125-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Cdk6 antibody PA2125-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish adrenal tissue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-21
Question
I see that the anti-Cdk6 antibody PA2125-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-10
Question
Will anti-Cdk6 antibody PA2125-2 work for WB with adrenal tissue?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
According to the expression profile of adrenal tissue, CDK6 is highly expressed in adrenal tissue. So, it is likely that anti-Cdk6 antibody PA2125-2 will work for WB with adrenal tissue.
Boster Scientific Support
Answered: 2019-08-23
Question
I have attached the WB image, lot number and protocol we used for adrenal tissue using anti-Cdk6 antibody PA2125-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
I would like to test anti-Cdk6 antibody PA2125-2 on mouse adrenal tissue for research purposes, then I may be interested in using anti-Cdk6 antibody PA2125-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The products we sell, including anti-Cdk6 antibody PA2125-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-05
Question
Can you help my question with product PA2125-2, anti-Cdk6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2125-2 anti-Cdk6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-04
Question
My team were content with the WB result of your anti-Cdk6 antibody. However we have observed positive staining in adrenal tissue ruffle. using this antibody. Is that expected? Could you tell me where is CDK6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
From what I have seen in literature, adrenal tissue does express CDK6. Generally CDK6 expresses in cytoplasm. nucleus. cell projection, ruffle. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-03-07
Question
We have seen staining in rat adrenal tissue. Any tips? Is anti-Cdk6 antibody supposed to stain adrenal tissue positively?
Z. Parker
Verified customer
Asked: 2018-05-22
Answer
From what I have seen in literature adrenal tissue does express CDK6. From what I have seen in Uniprot.org, CDK6 is expressed in adrenal tissue, tongue, brain, leukemic t-cell, among other tissues. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-22
Question
Does PA2125-2 anti-Cdk6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
It shows on the product datasheet, PA2125-2 anti-Cdk6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-07
Question
Is there a BSA free version of anti-Cdk6 antibody PA2125-2 available?
C. Krishna
Verified customer
Asked: 2014-12-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cdk6 antibody PA2125-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-19
Question
Is a blocking peptide available for product anti-Cdk6 antibody (PA2125-2)?
J. Brown
Verified customer
Asked: 2014-06-19
Answer
We do provide the blocking peptide for product anti-Cdk6 antibody (PA2125-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-06-19
Question
We are currently using anti-Cdk6 antibody PA2125-2 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
T. Evans
Verified customer
Asked: 2014-04-18
Answer
The anti-Cdk6 antibody (PA2125-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-18
