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Product Info Summary
| SKU: | MP00166 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody
SKU/Catalog Number
MP00166
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody catalog # MP00166. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00166)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GBB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
MP00166 is reactive to CDK2 in Human, Mouse, Rat
Applications
MP00166 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
78 kDa
Calculated molecular weight
26053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-CDK2 (Y15) expression in (1) HeLa treated with AP; (2) HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-CDK2 (Y15) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDK2 (Source: Uniprot.org, NCBI)
Gene Name
CDK2
Full Name
Cyclin-dependent kinase 2
Weight
26053 MW
Superfamily
protein kinase superfamily
Alternative Names
cdc2-related protein kinase; CDK2; cell devision kinase 2; Cell division protein kinase 2; cyclin-dependent kinase 2; EC 2.7.11; EC 2.7.11.22; p33 protein kinase; p33(CDK2) CDK2 CDKN2, p33(CDK2) cyclin dependent kinase 2 cyclin-dependent kinase 2|cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK2, check out the CDK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody (MP00166)
Hello CJ!
MP00166 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IL-2R%u03B1 up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell %u2026
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 %u2026
Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Liu X, Lin X, Mi Y, Li J, Zhang C. Oxid Med Cell Longev. 2018 Jan 9;2018:9390810. doi: 10.1155/2018/9390810. eCollection 2018. Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody
Question
I am interested in to test anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The products we sell, including anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-Phospho-CDK2 (Y15) Monoclonal antibody (MP00166)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
We do provide the blocking peptide for product anti-Phospho-CDK2 (Y15) Monoclonal antibody (MP00166). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-15
Question
We are currently using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The anti-Phospho-CDK2 (Y15) Monoclonal antibody (MP00166) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
Would MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
It shows on the product datasheet, MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
Our lab used your anti-Phospho-CDK2 (Y15) Monoclonal antibody for IP on cervix carcinoma a few years ago. I am using rat, and We intend to use the antibody for IHC next. We are interested in examining cervix carcinoma as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
I have checked the website and datasheets of our anti-Phospho-CDK2 (Y15) Monoclonal antibody and it appears that MP00166 has been validated on rat in both IP and IHC. Thus MP00166 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-22
Question
Our lab were well pleased with the WB result of your anti-Phospho-CDK2 (Y15) Monoclonal antibody. However we have been able to see positive staining in left lobe of thyroid gland cytoskeleton using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
Based on literature, left lobe of thyroid gland does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-04-12
Question
Is there a BSA free version of anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 available?
N. Wu
Verified customer
Asked: 2018-05-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-28
Question
Is this MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody reactive to the isotypes of CDK2?
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
The immunogen of MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody is A synthesized peptide derived from human CDK2 CDK2 is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. This protein can be regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-22
Question
Will anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 work for WB with leukemic t-cell?
W. Collins
Verified customer
Asked: 2017-06-06
Answer
According to the expression profile of leukemic t-cell, CDK2 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2017-06-06
Question
We have seen staining in mouse left lobe of thyroid gland. Any tips? Is anti-Phospho-CDK2 (Y15) Monoclonal antibody supposed to stain left lobe of thyroid gland positively?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
Based on literature left lobe of thyroid gland does express CDK2. Based on Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-26
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166. Please let me know if you require anything else.
A. Anderson
Verified customer
Asked: 2017-02-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-01
Question
I was wanting to use your anti-Phospho-CDK2 (Y15) Monoclonal antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
A. Yang
Verified customer
Asked: 2015-11-25
Answer
As indicated on the product datasheet, MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-25
Question
I see that the anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 works with WB, what is the protocol used to produce the result images on the product page?
B. Walker
Verified customer
Asked: 2015-06-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-22
Question
I have a question about product MP00166, anti-Phospho-CDK2 (Y15) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Krishna
Verified customer
Asked: 2014-04-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-28
