Anti-Cdk2 Antibody Picoband®

CDK2 antibody

Boster Bio Anti-Cdk2 Antibody Picoband® catalog # PB9534. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 22 publication(s).

Product Info Summary

SKU: PB9534
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Cdk2 Antibody Picoband®

View all CDK2 Antibodies

SKU/Catalog Number

PB9534

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cdk2 Antibody Picoband® catalog # PB9534. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cdk2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9534)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9534 is reactive to CDK2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

30 kDa

Calculated molecular weight

33930 MW, 30,035 MW

Background of CDK2

CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2). P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9534 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry, 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Positive Control

WB: human Jurlat whole cell, human HepG2 whole cell, human U2OS whole cell, human K562 whole cell, human T47D whole cell, human CACO-2 whole cell, human 293T whole cell
IHC: human invasive urothelial carcinoma of the bladder with squamous differentiation tissue
ICC/IF: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For CDK2 (Source: Uniprot.org, NCBI)

Gene Name

CDK2

Full Name

Cyclin-dependent kinase 2

Weight

33930 MW, 30,035 MW

Superfamily

protein kinase superfamily

Alternative Names

Cyclin-dependent kinase 2;2.7.11.22;Cell division protein kinase 2;p33 protein kinase;CDK2;CDKN2; CDK2 CDKN2, p33(CDK2) cyclin dependent kinase 2 cyclin-dependent kinase 2|cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK2, check out the CDK2 Infographic

CDK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9534 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene

Effects of tripterygium glycosides on restenosis following endovascular treatment

2,3,7,8-Tetrachlorodibenzo-p-dioxin Mediated Cleft palate by Mouse Embryonic Palate Mesenchymal Cells

Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest

Growth inhibition and cell-cycle arrest of human gastric cancer cells by Lycium barbarum polysaccharide

Dehydrocostus Lactone Suppresses Proliferation of Human Chronic Myeloid Leukemia Cells Through Bcr/Abl‐JAK/STAT Signaling Pathways

OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells

IL-2R%u03B1 up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell %u2026

Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 %u2026

Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro

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5 Customer Q&As for Anti-Cdk2 Antibody Picoband®

Question

My team were satisfied with the WB result of your anti-Cdk2 antibody. However we have been able to see positive staining in placenta cytoskeleton using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

Based on literature, placenta does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-09

Question

We have seen staining in mouse cervix carcinoma. Any tips? Is anti-Cdk2 antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

According to literature cervix carcinoma does express CDK2. According to Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-24

Question

We ordered your anti-Cdk2 antibody for WB on cervix carcinoma a few years ago. I am using mouse, and We intend to use the antibody for IHC-P next. you antibody examining cervix carcinoma as well as left lobe of thyroid gland in our next experiment. Do you have any suggestion on which antibody would work the best for IHC-P?

C. Jackson

Verified customer

Asked: 2019-02-15

Answer

I viewed the website and datasheets of our anti-Cdk2 antibody and it seems that PB9534 has been validated on mouse in both WB and IHC-P. Thus PB9534 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-02-15

Question

I was wanting to use using your anti-Cdk2 antibody for regulation of tp53 degradation studies. Has this antibody been tested with western blotting on hut whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

I appreciate your inquiry. This PB9534 anti-Cdk2 antibody is validated on rat liver tissue, kidney tissue, tissue lysate, human placenta tissue, hela whole cell lysate, hut whole cell lysate, jurkat whole cell lysate, a549 whole cell lysate, mouse testis tissue, lung cancer tissue. It is guaranteed to work for IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-10-17

Question

We are currently using anti-Cdk2 antibody PB9534 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-19

Answer

The anti-Cdk2 antibody (PB9534) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-19

Order DetailsPrice
PB9534

100μg

$370
PB9534-10ug

10μg sample (liquid)

$99
PB9534-Biotin

100 μg Biotin conjugated

$570
PB9534-Cy3

100 μg Cy3 conjugated

$570
PB9534-Dylight488

100 μg Dylight488 conjugated

$570
PB9534-Dylight550

100 μg Dylight550 conjugated

$570
PB9534-Dylight594

100 μg Dylight594 conjugated

$570
PB9534-FITC

100 μg FITC conjugated

$570
PB9534-HRP

100 μg HRP conjugated

$570
PB9534-APC

100 μg APC conjugated

$670
PB9534-PE

100 μg PE conjugated

$670
PB9534-iFluor647

100 μg iFluor647 conjugated

$670
PB9534-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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