Anti-Phospho-AFX (Ser197) FOXO4 Antibody

Product Name

Anti-Phospho-AFX (Ser197) FOXO4 Antibody

View all FOXO4 Antibodies

SKU/Catalog Number

P01819

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-AFX (Ser197) FOXO4 Antibody (Catalog # P01819). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-AFX (Ser197) FOXO4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01819)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P01819 is reactive to FOXO4 in Human, Mouse, Rat

Applications

P01819 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

53684 MW

Background of FOXO4

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Essers MA, et al. EMBO J 2004 Nov. 11.
Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 65kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) antibody P01819(left) or the same antibody preincubated with blocking peptide(right).

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Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) antibody P01819.

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Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) antibody P01819.

Gene/Protein Information For FOXO4 (Source: Uniprot.org, NCBI)

Alternative Names

AFX; AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7; Fork head domain transcription factor AFX1; forkhead box O4; forkhead box protein O4; MLLT7MGC120490; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7 FOXO4 AFX, AFX1, MLLT7 forkhead box O4 forkhead box protein O4|fork head domain transcription factor AFX1|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7

For more info on FOXO4, check out the FOXO4 Infographic

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In this infographic, you will see the following information for FOXO4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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