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Product Info Summary
| SKU: | PA1502 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BCAT2 Antibody Picoband®
SKU/Catalog Number
PA1502
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BCAT2 Antibody catalog # PA1502. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCAT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1502)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1502 is reactive to BCAT2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
44288 MW
Background of BCAT2
BCAT2, branched-chain aminotransferase 2, encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. The human BCAT2 gene is mapped to 19q13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1502 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A431 Whole Cell, SW620 Whole Cell, SMMC Whole Cell, JURKAT Whole Cell, CEM Whole Cell
IHC: Human Colon Cancer Tissue
Validation Images & Assay Conditions
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Anti-BCAT2 antibody, PA1502, Western blotting
All lanes: Anti BCAT2 (PA1502) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
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Anti-BCAT2 antibody, PA1502, IHC(P)
IHC(P): Human Colon Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For BCAT2 (Source: Uniprot.org, NCBI)
Gene Name
BCAT2
Full Name
Branched-chain-amino-acid aminotransferase, mitochondrial
Weight
44288 MW
Superfamily
class-IV pyridoxal-phosphate-dependent aminotransferase family
Alternative Names
Branched-chain-amino-acid aminotransferase, mitochondrial;BCAT (m);2.6.1.42;Placental protein 18;PP18;BCAT2;BCATM, BCT2, ECA40; BCAT2 BCAM, BCATM, BCT2, HVLI, PP18 branched chain amino acid transaminase 2 branched-chain-amino-acid aminotransferase, mitochondrial|branched chain amino-acid transaminase 2, mitochondrial|branched chain aminotransferase 2, mitochondrial|placental protein 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCAT2, check out the BCAT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCAT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BCAT2 Antibody Picoband® (PA1502)
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No publications found for PA1502
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Customer Q&As
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1 Customer Q&As for Anti-BCAT2 Antibody Picoband®
Question
We are currently using anti-BCAT2 antibody PA1502 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-BCAT2 antibody (PA1502) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
