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Product Info Summary
| SKU: | M08917 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-PHC3 Antibody (C-Term)
SKU/Catalog Number
M08917
Size
50 µl
Description
Boster Bio Anti-PHC3 Antibody (C-Term) (Catalog # M08917). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PHC3 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08917)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PHC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 819-850 amino acids from human PHC3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08917 is reactive to PHC3 in Human
Reconstitution
Calculated molecular weight
106162 Da
Background of PHC3
Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08917 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: SH-SY5Y whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: U-2OS whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 106 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M08917 staining PHC3 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For PHC3 (Source: Uniprot.org, NCBI)
Gene Name
PHC3
Full Name
Polyhomeotic-like protein 3
Weight
106162 Da
Alternative Names
Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3 PHC3 EDR3, HPH3 polyhomeotic homolog 3 polyhomeotic-like protein 3|early development regulator 3|early development regulatory protein 3|homolog of polyhomeotic 3|polyhomeotic like 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHC3, check out the PHC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHC3 Antibody (C-Term) (M08917)
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