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Product Info Summary
| SKU: | A05977Y14 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PFKFB2 (Y14) Antibody
SKU/Catalog Number
A05977Y14
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PFKFB2 (Y14) Antibody catalog # A05977Y14. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFKFB2 (Y14) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05977Y14)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human PFKFB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05977Y14 is reactive to PFKFB2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
58477 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05977Y14 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of PFKFB2 using Anti-PFKFB2 (Y14) Antibody (A05977Y14).
Immunohistochemistry (IHC) analyzes of PFKFB2 (Y14) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-PFKFB2 (Y14) Antibody A05977Y14
Western blot (WB) analysis of PFKFB2 (Y14) pAb at 1:500 dilutionLane1:A549 whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:Hela whole cell lysate(40ug)
Lane5:U-87MG whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 3. Western blotting validation for Anti-PFKFB2 (Y14) Antibody A05977Y14
Western blot (WB) analysis of PFKFB2 (Y14) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:The kidney tissue lysate of Mouse(40ug)
Lane4:The liver tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PFKFB2 (Source: Uniprot.org, NCBI)
Gene Name
PFKFB2
Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Weight
58477 MW
Alternative Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2;6PF-2-K/Fru-2,6-P2ase 2;PFK/FBPase 2;6PF-2-K/Fru-2,6-P2ase heart-type isozyme;6-phosphofructo-2-kinase;2.7.1.105;Fructose-2,6-bisphosphatase;3.1.3.46;PFKFB2; PFKFB2 PFK-2/FBPase-2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2|6PF-2-K/Fru-2,6-P2ASE heart-type isozyme|6PF-2-K/Fru-2,6-P2ase 2|PFK/FBPase 2|PFKFB, cardiac|fructose-2,6-bisphosphatase, cardiac isozyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKFB2, check out the PFKFB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKFB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PFKFB2 (Y14) Antibody (A05977Y14)
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