PFKFB2 Antibodies

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (PFKFB2)

Synthesis and degradation of fructose 2,6-bisphosphate. .

Gene Information

Human
Gene Name:	PFKFB2
Uniprot:	O60825
Entrez:	5208

Family

Belongs to:
No superfamily

Alternative Names

6PF-2-K/Fru-2,6-P2ase 2; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; DKFZp781D2217; fructose-2,6-bisphosphatase, cardiac isozyme; MGC138308,6PF-2-K/Fru-2,6-P2ase heart-type isozyme; MGC138310; PFK/FBPase 2; PFK-2/FBPase-2; PFKFB, cardiac

Molecular Weight

Mass (kDA):
58.477 kDA

Genomic Context

Human
Location:	1q32.1
Sequence:	1; NC_000001.11 (207053063..207081027)

Tissue Specificity

Heart.

Diseases

• Malignant Neoplasms
• Leukemia
• Acute Lymphocytic Leukemia
• L1 Acute Lymphoblastic Leukemia
• Malignant Neoplasm Of Prostate
• Neoplasm Metastasis
• Psoriasis
• Metastatic Malignant Neoplasm To The Lymph Node
• Schizophrenia
• Endometrial Polyp
• Carcinoma, Papillary
• Precursor T-cell Lymphoblastic Leukemia-lymphoma
• Carcinoma

• Fetal Growth Retardation
• Thyroid Neoplasm
• Acute Erythroblastic Leukemia
• Lymphatic Metastasis
• Delayed Hypersensitivity
• Prostatic Neoplasms
• Neoplasms

Interacting Proteins

• YWHAZ

Pathways

• Glycolysis
• Complement Activation
• Secretion
• Cell Activation
• Cell Migration
• Tricarboxylic Acid Cycle
• T Cell Aggregation
• T Cell Activation
• Immune Response
• Adaptive Immune Response
• Hypersensitivity
• Cell Cycle Arrest
• Embryo Development

• Chemotaxis
• Lymphocyte Chemotaxis
• Translation
• Cell Cycle
• T Cell Proliferation
• Cell Chemotaxis
• Cell Proliferation

PTMs

• Phosphorylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PFKFB2

Best Uses Of The PFKFB2 Marker

This article has useful information about PFKFB2 markings. This marker is an important part of many research projects. There are many uses and different applications for it. Continue reading to learn more about the best uses of the PFKFB2 marker. This product can be purchased by researchers all over the world and used in various scientific studies.

Anti-PFKFB2/P Pfk2 Antibody

There are many suppliers of Anti PFKFB2/P PfK 2 Car Antibody. However, which supplier is the best for your research needs? Boster Bio provides a variety anti-PFKFB2/P PfK 2 Car Antibody, including orthologs for the mouse, human, and rat proteins. The main benefits of the BosterBio Anti–PFKFB2/P Pfk2 Car Antibody are listed below.

The company's goal is to provide high-quality antibodies for researchers. They have antibodies that can be used to validate research against both known positive and negative samples. Their high affinity and specificity are key characteristics. They also reward customers who review their products early by offering product credits. Boster Bio offers a wide selection of ELISA products. Boster Bio has the right solution for you, whether you need to do ELISAs, IHC or use a kit for PCR.

Assay for glucose uptake colorimetric

A glucose uptake colorimetric test kit provides an easy and direct way to measure the amount of glucose that a cell can absorb. The glucose analog is phosphorylated using hexokinase, to form 2-DG6P. This form is inedible and accumulates in the cells. This method uses a recycling amplification reaction in order to detect glucose levels. AAT Bioquest's Screen Quest(tm), Colorimetric Glucose Uptake Assay Kit provides excellent quantitative measurements.

A fluorescent assay kit or colorimetric test kit can measure glucose uptake within cells. In colorimetric testing, glucose levels can drop to as low 10 pmol/well. Fluorometric glucose uptake tests, on the contrary, use a nonfluorescent technique. They are similar, but have some differences.

This glucose uptake assay can be used in many areas, including the diagnosis and treatment diabetes. In animal cell cultures, for example, glucose consumption can be measured using radiolabeled or fluorescent analogues. These methods are expensive and can pose health risks. Another option is to use glucose uptake colorimetric testing on adipocytes. The adipocytes in the 3T3-L1 cells were then differentiated and exposed to different glucose concentrations and insulin-stimulated environments.

A glucose-uptake and enzyme cycling method is another technique that uses colorimetric assays. This method is compatible to colorimetric assays on plates and with high-throughput automated workflows. Its flexibility and reliability make it an ideal tool for glucose metabolism research. This method is able to be applied in adherent and suspended cells that have been cultured in 96 well microtiter plates.

Human short hair RNA (shRNA) targeting KRT17

Transfection with shRNA targeting KRT17 in 293T cells resulted the generation of lentivectors, which bind to target genes. The transfected cells moved as predicted by the QCM Chemotaxis 96 well assay. The cells were cultured in 6-well plates at 6 x105 cells/dish to evaluate the effectiveness of the shRNAs. The cells were mechanically killed using a 100 ml tip. Finally, the lentivectors underwent examination under the microscope.

KRT17 has been knocked out in AGS cell lines. This demonstrated that a reduction or elimination of KRT17 increases phosphorylation Chk-2 (cell-cycle arrest proteins) and p53. These genes are involved in the regulation of cell-cycle arrest and growth. Chk-2 phosphorylates target genes in response to DNA damage. It repairs DNA damage by phosphorylating p53 when Chk2 does this.

Public-private partnerships are helping to advance research on human-specific shRNA. Human short hairRNA (shRNA), which targets KRT17, is a form shRNA that can target many gene-targeting proteins. It is capable of inhibiting the expression of several genes in the brain and is derived from a murine genome. The public-private collaboration aims at validating the shRNA library and producing siRNAs that are strand-specific.

We tested the phosphorylation KRT17 by using a human PKC array. This allows simultaneous analysis of 43 kinases as well as two related total proteins. The transfection efficiency of siRNA targeting KRT17 was optimized by using a lysis protein buffer containing 2.5 ml. The cells were harvested 48 h later and incubated overnight in nitrocellulose membranes at 4°C.

Human CDK2

Several studies have demonstrated the high phosphorylation of Human CDK2 substrates. These substrates can be found in many complexes involved in gene expression and cell division. These findings suggest CDK2 may phosphorylate these substrates in order to regulate their levels. This phosphorylation is mediated via direct phosphorylation or through cell cycle-specific mechanisms. This article explains the mechanisms of phosphorylation as well as the potential applications of this marker in the detection of CDK2 human cancer cells.

The PFKFB2 indicator is a sensitive and specific indicator of Human CDK2 activity in cell culture. The high phosphorylation level in CDK2 is accompanied by reduced protein synthesis, so a higher sensitivity reflects high CDK2 activity. These results suggest that CDK2 is essential for cell survival and proliferation. It is also possible that CDK2 phosphorylation can regulate the levels of some other proteins.

There are many drugs that can be used to reduce the activity of Human CDK2 cells cultured. PF-03814735 (SL-0101-1), SU9516 and SU9516 all inhibited CDK2 activities and decreased the activity triosephosphate Isomerase. The knockdown and inhibition of CDK2 by human cells slowed down cell growth and stopped cancer cells from reaching G1 phase. CDK2 inhibits hexokinase II. This enzyme was also knocked out, which halted the growth and proliferation of cancer cells.

Cyclin E/CDK2 phosphorylates USP37 and RB1 and promotes cell differentiation. It regulates cellular processes and complex substrate networks. AS mutations are common in mammalian CDKs. AS mutations are also possible in PFKFB2. Moreover, this enzyme phosphorylates BRCA2, which is a regulator of cyclin E in hESCs.

Human CDK4

The regulation and activation of SRC-3 is dependent on the PFKFB2 marker. It binds with the co-activator SRC-3 at the Ser857 residue. This facilitates the ER's binding. It also recruits the transcription factors ATF4 and AMPD1, which stimulate pyrimidine synthesis. SRC-3 promotes NAPDH, which, in turn, helps cells maintain a healthy intracellular redox environment.

PFK2 members play an important role in regulating glucose metabolism, which is essential for cell growth. These enzymes can also perform extra-glycolytic roles in cancer development. These roles require further research. The PFKFB2 signal may not be the best for cancer diagnosis because it lacks a specific signal to identify its role. However, it plays an intriguing role in regulation of glucose metabolism.

Although PFKFB2 isn't the only target for CDK inhibiters, it has been shown that it is effective against breast and cervical cancer. It is also important for prostate cancer treatment. CDK4 also plays a role in the regulation and maintenance of cell cycle. CDK inhibitors have been identified that target this enzyme. This means that targeted inhibition of CDK4 activity can potentially have a role in treating cancer.

This enzyme is controlled by multiple forms of covalent modifications, whereas PFKFB2 is not. Both PFK kinase forms are found primarily in cellular nucleus. The extraglycolytic mechanism by which cancer cells are stimulated by the nuclear PFKFB3 PFK protein stimulates them to proliferate. This receptor is not activated by PFKFB3 protein.