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Product Info Summary
| SKU: | A00053-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-CXCL12 Antibody
SKU/Catalog Number
A00053-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCL12 Antibody catalog # A00053-2. Tested in ELISA, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCL12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00053-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CXCL12 recombinant protein (Position: V24-M93).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00053-2 is reactive to CXCL12 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
25283 MW
Background of CXCL12
The stromal cell-derived factor 1 (SDF1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein that in humans is encoded by the CXCL12 gene on chromosome 10. This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00053-2 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 5 μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
IHC: human endometrial carcinoma tissue, rat spleen tissue, human tonsil tissue, human liver cancer tissue, human lymphoma tissue, mouse spleen tissue
IF: human hepatitis tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of human hepatitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 9. IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 10. IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CXCL12 (Source: Uniprot.org, NCBI)
Gene Name
CXCL12
Full Name
Stromal cell-derived factor 1
Weight
25283 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; SDF-1-beta (3-72); SDF-1-alpha (3-67); CXCL12; SDF1; SDF1A; SDF1B CXCL12 IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1 C-X-C motif chemokine ligand 12 stromal cell-derived factor 1|chemokine (C-X-C motif) ligand 12|intercrine reduced in hepatomas|pre-B cell growth-stimulating factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL12, check out the CXCL12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCL12 Antibody (A00053-2)
Hello CJ!
A00053-2 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF‑1α expression in vivo
Treatment of radiation‑induced acute intestinal injury with bone marrow‑derived mesenchymal stem cells
CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma
Selective tropism of liver stem cells to hepatocellular carcinoma in vivo
Angelica sinensis Polysaccharides Ameliorate Stress-Induced Premature Senescence of Hematopoietic Cell via Protecting Bone Marrow Stromal Cells from Oxidative Injuries Caused by 5-Fluorouracil
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Matrix elasticity-modified scaffold loaded with SDF-1α improves the in situ regeneration of segmental bone defect in rabbit radius
Estrogen-replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing SDF-1 and estrogen receptor expression
Role of SDF-1/CXCR4 and cytokines in the development of ovary injury in chemotherapy drug induced premature ovarian failure mice
SDF-1α/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells towards ischemic brain lesion in a rat model
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CXCL12 Antibody
Question
Here is the WB image, lot number and protocol we used for liver using anti-CXCL12 antibody A00053-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
We have seen staining in mouse liver. Any tips? Is anti-CXCL12 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Based on literature liver does express CXCL12. Based on Uniprot.org, CXCL12 is expressed in myometrium, fetal brain, fetal heart heart, fibroblast, liver, thymus uterus, brain, among other tissues. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-06
Question
Our lab used your anti-CXCL12 antibody for IHC on myometrium in a previous project. I am using rat, and I plan to use the antibody for ELISA next. Our lab want to know about examining myometrium as well as fetal brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
I looked at the website and datasheets of our anti-CXCL12 antibody and it seems that A00053-2 has been validated on rat in both IHC and ELISA. Thus A00053-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-12
Question
Is this A00053-2 anti-CXCL12 antibody reactive to the isotypes of CXCL12?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
The immunogen of A00053-2 anti-CXCL12 antibody is E. coli-derived human CXCL12 recombinant protein (Position: V24-M93). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-10
Question
Our team were satisfied with the WB result of your anti-CXCL12 antibody. However we have observed positive staining in fibroblast secreted. using this antibody. Is that expected? Could you tell me where is CXCL12 supposed to be expressed?
A. Gonzalez
Verified customer
Asked: 2019-10-02
Answer
From what I have seen in literature, fibroblast does express CXCL12. Generally CXCL12 expresses in secreted. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-02
Question
I was wanting to use your anti-CXCL12 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
You can see on the product datasheet, A00053-2 anti-CXCL12 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CXCL12 antibody A00053-2. Let me know if you need anything else.
N. Rodriguez
Verified customer
Asked: 2019-04-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-18
Question
Does anti-CXCL12 antibody A00053-2 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
According to the expression profile of liver, CXCL12 is highly expressed in liver. So, it is likely that anti-CXCL12 antibody A00053-2 will work for WB with liver.
Boster Scientific Support
Answered: 2018-01-01
Question
I was wanting to use to test anti-CXCL12 antibody A00053-2 on rat liver for research purposes, then I may be interested in using anti-CXCL12 antibody A00053-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
The products we sell, including anti-CXCL12 antibody A00053-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-07
Question
Is there a BSA free version of anti-CXCL12 antibody A00053-2 available?
R. Li
Verified customer
Asked: 2017-08-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CXCL12 antibody A00053-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-11
Question
I see that the anti-CXCL12 antibody A00053-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-04
Question
Will A00053-2 anti-CXCL12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
You can see on the product datasheet, A00053-2 anti-CXCL12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-03
Question
We are currently using anti-CXCL12 antibody A00053-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
A. Walker
Verified customer
Asked: 2015-12-09
Answer
The anti-CXCL12 antibody (A00053-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-09
Question
My question regarding product A00053-2, anti-CXCL12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Williams
Verified customer
Asked: 2015-10-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00053-2 anti-CXCL12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-10-15
Question
We need using your anti-CXCL12 antibody for positive regulation of cell migration studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
L. Collins
Verified customer
Asked: 2014-11-04
Answer
We appreciate your inquiry. This A00053-2 anti-CXCL12 antibody is tested on human tonsil tissue, placenta tissue, a431 whole cell lysate, endometrial carcinoma tissue, mouse spleen tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-11-04
Question
Is a blocking peptide available for product anti-CXCL12 antibody (A00053-2)?
H. Rodriguez
Verified customer
Asked: 2013-12-09
Answer
We do provide the blocking peptide for product anti-CXCL12 antibody (A00053-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-09
