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Product Info Summary
| SKU: | M03926 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-PERP Monoclonal Antibody
SKU/Catalog Number
M03926
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PERP Monoclonal Antibody catalog # M03926. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PERP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03926)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFDD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PERP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03926 is reactive to PERP in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
46 kDa
Calculated molecular weight
55990 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03926 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of PERP expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PERP (Source: Uniprot.org, NCBI)
Gene Name
PERP
Full Name
p53 apoptosis effector related to PMP-22
Weight
55990 MW
Superfamily
TMEM47 family
Alternative Names
Perilipin-1;Lipid droplet-associated protein;PLIN1;PERI, PLIN; Perp|1110017A08Rik, KCP, KCP1, KRTC, KRTCAP1, PIG, PIGPC1, THW|PERP, TP53 apoptosis effector|p53 apoptosis effector related to PMP-22|KCP-1|keratinocyte-associated protein 1|p53 apoptosis effector related to Pmp22|p53 apoptosis-associated target
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PERP, check out the PERP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PERP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PERP Monoclonal Antibody (M03926)
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No publications found for M03926
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PERP Monoclonal Antibody
Question
Would anti-PERP Monoclonal antibody M03926 work for ICC with pancreas?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
According to the expression profile of pancreas, PERP is highly expressed in pancreas. So, it is likely that anti-PERP Monoclonal antibody M03926 will work for ICC with pancreas.
Boster Scientific Support
Answered: 2020-03-12
Question
What is the amount of the biotin or HRP secondary antibody for the M03926?
Verified customer
Asked: 2019-09-12
Answer
For the Anti-PERP Monoclonal Antibody (M03926) secondary antibody would be the smallest size 0.5ml.
Boster Scientific Support
Answered: 2019-09-12
Question
Would M03926 anti-PERP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can see on the product datasheet, M03926 anti-PERP Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-PERP Monoclonal antibody M03926. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
I was wanting to use your anti-PERP Monoclonal antibody for ICC for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?
Z. Wu
Verified customer
Asked: 2017-10-30
Answer
It shows on the product datasheet, M03926 anti-PERP Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-30
Question
We are currently using anti-PERP Monoclonal antibody M03926 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
B. Singh
Verified customer
Asked: 2016-07-15
Answer
The anti-PERP Monoclonal antibody (M03926) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-15
Question
Does anti-PERP Monoclonal antibody M03926 work on horse IF with keratinocyte?
C. Li
Verified customer
Asked: 2015-12-08
Answer
Our lab technicians have not validated anti-PERP Monoclonal antibody M03926 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PERP Monoclonal antibody M03926 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse keratinocyte in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-08
