Anti-PEDF Monoclonal Antibody

Serpin F1/PEDF antibody

Boster Bio Anti-PEDF Monoclonal Antibody catalog # M02034. Tested in WB, Flow Cytometry applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M02034
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-PEDF Monoclonal Antibody

View all Serpin F1/PEDF Antibodies

SKU/Catalog Number

M02034

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PEDF Monoclonal Antibody catalog # M02034. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PEDF Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02034)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFED-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PEDF Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02034 is reactive to SERPINF1 in Human

Applications

M02034 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

13157 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For SERPINF1 (Source: Uniprot.org, NCBI)

Gene Name

SERPINF1

Full Name

Pigment epithelium-derived factor

Weight

13157 MW

Superfamily

serpin family

Alternative Names

Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1 SERPINF1 EPC-1, OI12, OI6, PEDF, PIG35 serpin family F member 1 pigment epithelium-derived factor|alpha-2 antiplasmin|cell proliferation-inducing gene 35 protein|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1|testis tissue sperm-binding protein Li 70n

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SERPINF1, check out the SERPINF1 Infographic

SERPINF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SERPINF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02034 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Clinicopathological Features and Prognosis of Papillary Thyroid Microcarcinoma for Surgery and Relationships with the BRAFV600E Mutational Status and Expression of Angiogenic Factors

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4 Customer Q&As for Anti-PEDF Monoclonal Antibody

Question

What sample was tested in WB and IHC applications with M02034?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of PEDF expression in K562 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

Is there a BSA free version of anti-PEDF Monoclonal antibody M02034 available?

Verified Customer

Verified customer

Asked: 2018-04-06

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PEDF Monoclonal antibody M02034 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-04-06

Question

Will M02034 anti-PEDF Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

As indicated on the product datasheet, M02034 anti-PEDF Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-06

Question

We are currently using anti-PEDF Monoclonal antibody M02034 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

N. Parker

Verified customer

Asked: 2014-04-04

Answer

The anti-PEDF Monoclonal antibody (M02034) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-04

Order DetailsPrice
M02034

100μL

$370
M02034-30ul

30μL sample

$99
M02034-carrier-free-1ml

Carrier Free

$2590

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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