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Key=created_at, value=2017-01-22 13:32:45
Key=updated_at, value=2024-10-04 05:03:29
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Key=name, value=Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®
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Key=meta_title, value=Connexin 45/GJA7/GJC1 Antibody A08562
Key=meta_description, value=Boster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 4. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate b
Key=small_image_label, value=Figure 4. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate b
Key=thumbnail_label, value=Figure 4. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate b
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Key=size, value=100 μg/vial
Key=protein_name, value=Gap junction gamma-1 protein
Key=gene_full_name, value=Gap junction gamma-1 protein
Key=uniprot_id, value=P36383
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Key=predicted_reactivity
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Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=45470 MW
Key=sample_size_available, value=30ug for $99, contact us for details
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Key=product_category, value=Primary Antibodies
Key=research_category, value=Cell Adhesion, Cytoskeleton/ECM, Signal Transduction
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Key=description, value=Boster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Gap junction gamma-1 protein (GJC1), also known as gap junction alpha-7 protein (GJA7) or connexin 45 (Cx45), is a protein that in humans is encoded by the GJC1 gene. The International Radiation Hybrid Mapping Consortium mapped the GJA7 gene to chromosome 17q21.31. This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell.
Key=gene_name, value=GJC1
Key=synonyms, value=Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7;
Key=application_details, value=Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human

Key=subcellular_localization, value= Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Key=tissue_specificity, value=Ubiquitously expressed.
Key=protein_function, value= One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 45/GJA7, identical to the related mouse and rat sequences.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=45 kDa
Key=custom_attribute_2, value=WB: rat testis tissue
IHC: human intestinal cancer tissue, rat skeletal muscle tissue, mouse cardiac muscle tissue, human placneta tissue
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Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®

Connexin 45/GJC1 antibody

Boster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A08562
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®

View all Connexin 45/GJC1 Antibodies

SKU/Catalog Number

A08562

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08562)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 45/GJA7, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A08562 is reactive to GJC1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

45 kDa

Calculated molecular weight

45470 MW

Background of Connexin 45/GJC1

Gap junction gamma-1 protein (GJC1), also known as gap junction alpha-7 protein (GJA7) or connexin 45 (Cx45), is a protein that in humans is encoded by the GJC1 gene. The International Radiation Hybrid Mapping Consortium mapped the GJA7 gene to chromosome 17q21.31. This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A08562 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human

Positive Control

WB: rat testis tissue
IHC: human intestinal cancer tissue, rat skeletal muscle tissue, mouse cardiac muscle tissue, human placneta tissue

Validation Images & Assay Conditions

Gene/Protein Information For GJC1 (Source: Uniprot.org, NCBI)

Gene Name

GJC1

Full Name

Gap junction gamma-1 protein

Weight

45470 MW

Superfamily

connexin family

Alternative Names

Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7; GJC1 CX45, GJA7 gap junction protein gamma 1 gap junction gamma-1 protein|CTC-296K1.4|connexin-45|gap junction alpha-7 protein|gap junction protein, gamma 1, 45kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GJC1, check out the GJC1 Infographic

GJC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GJC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A08562 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats

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3 Customer Q&As for Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®

Question

See attached the WB image, lot number and protocol we used for neocortex using anti-Connexin 45/GJA7/GJC1 antibody A08562. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

I see that the anti-Connexin 45/GJA7/GJC1 antibody A08562 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-22

Question

I was wanting to use your anti-Connexin 45/GJA7/GJC1 antibody for IHC for rat neocortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat neocortex identification?

W. Jackson

Verified customer

Asked: 2019-03-05

Answer

As indicated on the product datasheet, A08562 anti-Connexin 45/GJA7/GJC1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat neocortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-05

Order DetailsPrice
A08562

100μg

$370
A08562-10ug

10μg sample (liquid)

$99
A08562-Biotin

100 μg Biotin conjugated

$570
A08562-Cy3

100 μg Cy3 conjugated

$570
A08562-Dylight488

100 μg Dylight488 conjugated

$570
A08562-Dylight550

100 μg Dylight550 conjugated

$570
A08562-Dylight594

100 μg Dylight594 conjugated

$570
A08562-FITC

100 μg FITC conjugated

$570
A08562-HRP

100 μg HRP conjugated

$570
A08562-APC

100 μg APC conjugated

$670
A08562-PE

100 μg PE conjugated

$670
A08562-iFluor647

100 μg iFluor647 conjugated

$670
A08562-carrier-free

Carrier Free

$370

More conjugates/formats

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A08562
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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