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Product Info Summary
| SKU: | A08562 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®
View all Connexin 45/GJC1 Antibodies
SKU/Catalog Number
A08562
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08562)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 45/GJA7, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A08562 is reactive to GJC1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
45470 MW
Background of Connexin 45/GJC1
Gap junction gamma-1 protein (GJC1), also known as gap junction alpha-7 protein (GJA7) or connexin 45 (Cx45), is a protein that in humans is encoded by the GJC1 gene. The International Radiation Hybrid Mapping Consortium mapped the GJA7 gene to chromosome 17q21.31. This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08562 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat testis tissue
IHC: human intestinal cancer tissue, rat skeletal muscle tissue, mouse cardiac muscle tissue, human placneta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 4. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 45/GJA7 Antibody (A08562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of rat skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 45/GJA7 Antibody (A08562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 45/GJA7 Antibody (A08562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Connexin 45/GJA7 was detected in paraffin-embedded section of human placneta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 45/GJA7 Antibody (A08562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 45/GJA7 antigen affinity purified polyclonal antibody (Catalog # A08562) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 45/GJA7 at approximately 45KD. The expected band size for Connexin 45/GJA7 is at 45KD.
Protein Target Info & Infographic
Gene/Protein Information For GJC1 (Source: Uniprot.org, NCBI)
Gene Name
GJC1
Full Name
Gap junction gamma-1 protein
Weight
45470 MW
Superfamily
connexin family
Alternative Names
Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7; GJC1 CX45, GJA7 gap junction protein gamma 1 gap junction gamma-1 protein|CTC-296K1.4|connexin-45|gap junction alpha-7 protein|gap junction protein, gamma 1, 45kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJC1, check out the GJC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® (A08562)
Hello CJ!
A08562 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats
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Customer Q&As
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3 Customer Q&As for Anti-Connexin 45/GJA7/GJC1 Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for neocortex using anti-Connexin 45/GJA7/GJC1 antibody A08562. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-16
Question
I see that the anti-Connexin 45/GJA7/GJC1 antibody A08562 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-22
Question
I was wanting to use your anti-Connexin 45/GJA7/GJC1 antibody for IHC for rat neocortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat neocortex identification?
W. Jackson
Verified customer
Asked: 2019-03-05
Answer
As indicated on the product datasheet, A08562 anti-Connexin 45/GJA7/GJC1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat neocortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-05
