Click image to see more details
Product Info Summary
| SKU: | EK0896 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PEDF/SerpinF1 ELISA Kit PicoKine®
View all Serpin F1/PEDF ELISA kits
SKU/Catalog Number
EK0896
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PEDF/SerpinF1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PEDF/SerpinF1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0896)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: M1-P418
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0896 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0896 is reactive to SERPINF1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0896 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin F1/PEDF
Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0896-CAP | Anti-Human SERPINF1 Pre-coated 96-well strip microplate | 1 |
| EK0896-ST | Human SERPINF1 Standard | 2 vials, 10 ng/tube |
| EK0896-DA | Human SERPINF1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.006 | 0.011 | 0.240 | 0.408 | 0.659 | 1.021 | 1.410 | 1.956 |
Data Example Images
Click image to see more details
Human PEDF/SerpinF1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin F1/PEDF, Dilution ratio of 1:10000, concentration in serum and plasma is 14.2-19.1 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PEDF/SerpinF1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 286 | 780 | 2612 | 271 | 802 | 2714 |
| Standard deviation | 19.16 | 44.46 | 104 48 | 19.51 | 53.73 | 130.27 |
| CV (%) | 6.7% | 5.7% | 4% | 7.2% | 6.7% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin F1/PEDF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 286 | 285 | 271 | 293 | 284 | 13.05 | 4.6% |
| Sample 2 | 780 | 796 | 821 | 805 | 801 | 33.62 | 4.2% |
| Sample 3 | 2612 | 2744 | 2752 | 2653 | 2690 | 104.92 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For SERPINF1 (Source: Uniprot.Org, NCBI)
Gene Name
SERPINF1
Full Name
Pigment epithelium-derived factor
Weight
46312 MW
Superfamily
serpin family
Alternative Names
Pigment epithelium-derived factor;PEDF;Cell proliferation-inducing gene 35 protein;EPC-1;Serpin F1;SERPINF1;PEDF;PIG35; SERPINF1 EPC-1, OI12, OI6, PEDF, PIG35 serpin family F member 1 pigment epithelium-derived factor|alpha-2 antiplasmin|cell proliferation-inducing gene 35 protein|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1|testis tissue sperm-binding protein Li 70n
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SERPINF1, check out the SERPINF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SERPINF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PEDF/SerpinF1 ELISA Kit PicoKine® (EK0896)
Hello CJ!
EK0896 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pigment Epithelium-Derived Factor Affects Angiogenesis in Celiac Disease
Polyethylene glycol-modified pigment epithelial-derived factor: new prospects for treatment of retinal neovascularization
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human PEDF/SerpinF1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human PEDF/SerpinF1 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Human PEDF/SerpinF1 ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-10-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-10-16
Question
Q: if the enzyme conjugated Serpin F1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin F1 antigen?
W. Singh
Verified customer
Asked: 2020-01-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-01-14
Question
How much concentration of sodium azide or thimerosal and 2-mercaptoethanol (or other toxic substances), if it is contained in EK0896?
Verified customer
Asked: 2019-10-28
Answer
The Human PEDF/SerpinF1 ELISA Kit PicoKine EK0896 have Recombinant standard - sodium azide 0.02mg/vial biotinylated antibody, sample, antibody, and abc diluent buffer - sodium azide 0.02%.
Boster Scientific Support
Answered: 2019-10-29
Question
Does EK0896 contain the protein derived from Baculovirus expression system?
Verified customer
Asked: 2019-10-28
Answer
The immunogen standard in the Human PEDF/SerpinF1 ELISA Kit PicoKine EK0896 is synthesized peptide.
Boster Scientific Support
Answered: 2019-10-29
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Serpin F1 using the EK0896 Human Serpin F1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-06-12
Question
Does the PBS preparation require the necessary addition of Na2HPO4, KCl and KH2PO4 when using EK0896?
Verified customer
Asked: 2019-04-12
Answer
It does not require the extra addition of Na2HPO4, KCl and KH2PO4 when using the Human PEDF/SerpinF1 ELISA Kit PicoKine® (EK0896).
Boster Scientific Support
Answered: 2019-04-15
Question
According to the EK0896 manual on preparation before the experiment to prepare the wash buffer, it is supposed to dilute the wash buffer powder in 1L of distilled water, however in the kit components there is no mention of this wash buffer powder. How should the solution be prepared?
Verified customer
Asked: 2019-04-04
Answer
The washing buffer (neutral PBS or TBS) for the Human PEDF/SerpinF1 ELISA Kit PicoKine™ (EK0896) can be prepared in one of the following ways: -Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2. -Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7. or it can be purchased: https://www.bosterbio.com/pbs-buffer-ar0030-e-boster.html
Boster Scientific Support
Answered: 2019-04-04
Question
Are the antibodies in the EK0896 kit monoclonal or polyclonal?
Verified customer
Asked: 2019-03-28
Answer
The capture antibody of the Human PEDF/SerpinF1 ELISA Kit PicoKine™ (EK0896) is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat.
Boster Scientific Support
Answered: 2019-03-29
Question
Are the antibodies in the EK0896 kit monoclonal or polyclonal?
Verified customer
Asked: 2019-03-28
Answer
The capture antibody of the Human PEDF/SerpinF1 ELISA Kit PicoKine™ (EK0896) is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat.
Boster Scientific Support
Answered: 2019-03-29
Question
Q: can I use citrate plasma as samples in Human Serpin F1 Picokine® ELISA Kit (Catalog # EK0896)?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Serpin F1 causing disruption of its protein structure. Serpin F1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Serpin F1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-09
Question
Q: can you recommend the dilution ratio of serum samples for detection of Serpin F1 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Serpin F1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
L. Phillips
Verified customer
Asked: 2018-06-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-06-14
Question
Q: which procedure should I follow in order to thaw whole blood sample for Serpin F1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin F1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-05-18
Question
Greetings, I hope things are going well. My colleague Sukhvinder Deo here at Abcam referred me to you. One of our customers has had success with your ELISA product EK0896 for detection of human PEDF/SERPINF1. https://www.bosterbio.com/human-pedf-serpinf1-picokine-trade-elisa-kit-ek0896-boster.html They are interested in purchasing more kits for QC of their product but have questions they hope we can answer before they move forward with a large purchase. Their questions pertain to consistency of performance over time, lot to lot. Can you please direct these questions to the people at Boster who can answer them? I found the answer to question #1 from the datasheet. 1) Are the antibodies in the kit monoclonal or polyclonal? Capture Antibody | Detection Antibody: monoclonal antibody from mouse|polyclonal antibody from goat 2) If they are polyclonal, what is the availability of the antibodies over the next 40-50 kits? How many kits can be manufactured from a lot of antibody? Have you ever switched lots? 3) What quality control assays are performed on the kits/reagents prior to shipping? Are any quality systems (ex. CAPA management) in place? Kind Regards, Tom
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
Please see the following answers. 1) Are the antibodies in the kit monoclonal or polyclonal? Capture Antibody | Detection Antibody Capture Antibody: polyclonal antibody from goat Detection Antibody: polyclonal antibody from goat 2) If they are polyclonal, what is the availability of the antibodies over the next 40-50 kits? How many kits can be manufactured from a lot of antibody? Have you ever switched lots? We have enough capture and detection antibodies for 40-50 kits in stock. It takes around one month for our lab to prepare 40-50 kits from the same lot. 100kits can be manufactured from a lot of antibody. We will use antibody from a new lot for your order of 40-50kits. 3) What quality control assays are performed on the kits/reagents prior to shipping? Are any quality systems (ex. CAPA management) in place? We thoroughly validate each lot using biologically relevant matrices such as serum, plasma. Validation testing for each lot of ELISA Kits includes intra- and inter-assay precision, reproducibility, assay linearity and stability. ISO9001 quality system including CAPA management is in place.
Boster Scientific Support
Answered: 2018-02-12
Question
Q: Can Serpin F1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Maharaj
Verified customer
Asked: 2018-01-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-01-15
Question
Q: how to analyze ELISA data? I have performed Serpin F1 level in plasma.
P. Puri
Verified customer
Asked: 2017-06-24
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-06-24
Question
Q: What is the optimal O.D. value for Serpin F1 ELISA kit? I performed your Serpin F1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Serpin F1 even though the O.D. values are not very high?
Z. Edwards
Verified customer
Asked: 2016-02-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin F1 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of Serpin F1 that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2016-02-10
