Click image to see more details
Product Info Summary
| SKU: | A01906-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-PDHA1/Pdh E1Alpha Antibody
View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies
SKU/Catalog Number
A01906-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PDHA1/Pdh E1Alpha Antibody catalog # A01906-1. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDHA1/Pdh E1Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01906-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PDHA1. AA range:314-363
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01906-1 is reactive to PDHA1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
39 kDa
Calculated molecular weight
43296 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01906-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000
Protein Target Info & Infographic
Gene/Protein Information For PDHA1 (Source: Uniprot.org, NCBI)
Gene Name
PDHA1
Full Name
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Weight
43296 MW
Alternative Names
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;1.2.4.1;PDHE1-A type I;PDHA1;PHE1A; PDHA1 PDHA, PDHAD, PDHCE1A, PHE1A pyruvate dehydrogenase E1 subunit alpha 1 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|PDHE1-A type I|pyruvate dehydrogenase (lipoamide) alpha 1|pyruvate dehydrogenase E1 alpha 1 subunit|pyruvate dehydrogenase alpha 1|pyruvate dehydrogenase complex, E1-alpha polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDHA1, check out the PDHA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDHA1/Pdh E1Alpha Antibody (A01906-1)
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No publications found for A01906-1
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Customer Q&As
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5 Customer Q&As for Anti-PDHA1/Pdh E1Alpha Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus using anti-PDHA1/Pdh E1Alpha antibody A01906-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
I see that the anti-PDHA1/Pdh E1Alpha antibody A01906-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-26
Question
Is this A01906-1 anti-PDHA1/Pdh E1Alpha antibody reactive to the isotypes of PDHA1?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
The immunogen of A01906-1 anti-PDHA1/Pdh E1Alpha antibody is Synthesized peptide derived from the C-terminal region of human PDHA1. at AA rangle: 290-370. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-31
Question
Will A01906-1 anti-PDHA1/Pdh E1Alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
You can see on the product datasheet, A01906-1 anti-PDHA1/Pdh E1Alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-05
Question
Will anti-PDHA1/Pdh E1Alpha antibody A01906-1 work on zebrafish WB with cervix carcinoma erythroleukemia?
K. Miller
Verified customer
Asked: 2014-03-27
Answer
Our lab technicians have not validated anti-PDHA1/Pdh E1Alpha antibody A01906-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PDHA1/Pdh E1Alpha antibody A01906-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-27
