Click image to see more details
Product Info Summary
| SKU: | A04469 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rabbit |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PDC-E2 Monoclonal Antibody
View all Pyruvate Dehydrogenase E2 Antibodies
SKU/Catalog Number
A04469
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PDC-E2 Monoclonal Antibody catalog # A04469. Tested in WB applications. This antibody reacts with Human, Rabbit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDC-E2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04469)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
3F8
Isotype
IgG
Immunogen
Purified recombinant human PDC-E2 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04469 is reactive to DLAT in Human, Rabbit
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
68997 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04469 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using PDC-E2 Monoclonal antibody against Jurkat, A549, U251, F9, LNCAP, HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DLAT (Source: Uniprot.org, NCBI)
Gene Name
DLAT
Full Name
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Weight
68997 MW
Superfamily
2-oxoacid dehydrogenase family
Alternative Names
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial;2.3.1.12;70 kDa mitochondrial autoantigen of primary biliary cirrhosis;PBC;Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex;M2 antigen complex 70 kDa subunit;Pyruvate dehydrogenase complex component E2;PDC-E2;PDCE2;DLAT;DLTA; DLAT DLTA, E2, PBC, PDC-E2, PDCE2 dihydrolipoamide S-acetyltransferase dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial|70 kDa mitochondrial auto of primary biliary cirrhosis|E2 component of pyruvate dehydrogenase complex|M2 complex 70 kDa subunit|dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex|pyruvate dehydrogenase complex component E2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLAT, check out the DLAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDC-E2 Monoclonal Antibody (A04469)
Hello CJ!
No publications found for A04469
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PDC-E2 Monoclonal Antibody ?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PDC-E2 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-PDC-E2 Monoclonal Antibody
Question
Is this A04469 anti-PDC-E2 Monoclonal antibody reactive to the isotypes of DLAT?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The immunogen of A04469 anti-PDC-E2 Monoclonal antibody is Purified recombinant human PDC-E2 protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-11
Question
I see that the anti-PDC-E2 Monoclonal antibody A04469 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-19
Question
Would anti-PDC-E2 Monoclonal antibody A04469 work on primate WB with liver?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Our lab technicians have not tested anti-PDC-E2 Monoclonal antibody A04469 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PDC-E2 Monoclonal antibody A04469 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
Does anti-PDC-E2 Monoclonal antibody A04469 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
According to the expression profile of kidney, DLAT is highly expressed in kidney. So, it is likely that anti-PDC-E2 Monoclonal antibody A04469 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-05-30
Question
Does A04469 anti-PDC-E2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
You can see on the product datasheet, A04469 anti-PDC-E2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-23
Question
We are currently using anti-PDC-E2 Monoclonal antibody A04469 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rabbit. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The anti-PDC-E2 Monoclonal antibody (A04469) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-09
Question
Here is the WB image, lot number and protocol we used for kidney using anti-PDC-E2 Monoclonal antibody A04469. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-06
Question
Can you help my question with product A04469, anti-PDC-E2 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04469 anti-PDC-E2 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-14
Question
We need to test anti-PDC-E2 Monoclonal antibody A04469 on human kidney for research purposes, then I may be interested in using anti-PDC-E2 Monoclonal antibody A04469 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Singh
Verified customer
Asked: 2017-08-03
Answer
The products we sell, including anti-PDC-E2 Monoclonal antibody A04469, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-PDC-E2 Monoclonal antibody A04469. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-01
Question
I was wanting to use your anti-PDC-E2 Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
K. Dhar
Verified customer
Asked: 2015-01-22
Answer
You can see on the product datasheet, A04469 anti-PDC-E2 Monoclonal antibody has been validated for WB on human, rabbit tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-22
Question
Is a blocking peptide available for product anti-PDC-E2 Monoclonal antibody (A04469)?
M. Anderson
Verified customer
Asked: 2014-07-24
Answer
We do provide the blocking peptide for product anti-PDC-E2 Monoclonal antibody (A04469). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-24
Question
Do you have a BSA free version of anti-PDC-E2 Monoclonal antibody A04469 available?
R. Krishna
Verified customer
Asked: 2013-05-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PDC-E2 Monoclonal antibody A04469 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-06
