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Product Info Summary
| SKU: | A10537 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-AIBP NAXE Antibody
SKU/Catalog Number
A10537
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-AIBP NAXE Antibody (Catalog # A10537). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AIBP NAXE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10537)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human AIBP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10537 is reactive to NAXE in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
31675 MW
Background of NAXE
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX By similarity. HAMAP-Rule MF_03159
Ritter M., Genomics 79:693-702(2002).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10537 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, using AIBP antibody A10537.
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Immunofluorescence analysis of A549 cells, using AIBP antibody A10537.
Protein Target Info & Infographic
Gene/Protein Information For NAXE (Source: Uniprot.org, NCBI)
Gene Name
NAXE
Full Name
NAD(P)H-hydrate epimerase
Weight
31675 MW
Superfamily
NnrE/AIBP family
Alternative Names
NAD (P)H-hydrate epimerase ;5.1.99.6;Apolipoprotein A-I-binding protein ;AI-BP ;NAD (P)HX epimerase ;YjeF N-terminal domain-containing protein 1;YjeF_N1;NAXE ;AIBP , APOA1BP, YJEFN1; NAXE AIBP, APOA1BP, PEBEL, YJEFN1 NAD(P)HX epimerase NAD(P)H-hydrate epimerase|AI-BP|apoA-I binding protein|apolipoprotein A-I-binding protein|yjeF N-terminal domain-containing protein 1|yjeF_N1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAXE, check out the NAXE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAXE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AIBP NAXE Antibody (A10537)
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No publications found for A10537
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Customer Q&As
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7 Customer Q&As for Anti-AIBP NAXE Antibody
Question
Is this A10537 anti-AIBP antibody reactive to the isotypes of NAXE?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The immunogen of A10537 anti-AIBP antibody is Synthesized peptide derived from internal of human AIBP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-30
Question
I was wanting to use to test anti-AIBP antibody A10537 on human left uterine tube for research purposes, then I may be interested in using anti-AIBP antibody A10537 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The products we sell, including anti-AIBP antibody A10537, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-31
Question
Can you help my question with product A10537, anti-AIBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Miller
Verified customer
Asked: 2019-09-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10537 anti-AIBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-25
Question
I was wanting to use your anti-AIBP antibody for IF for human left uterine tube on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human left uterine tube identification?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
It shows on the product datasheet, A10537 anti-AIBP antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human left uterine tube in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-01
Question
Does anti-AIBP antibody A10537 work for IF with left uterine tube?
C. Dhar
Verified customer
Asked: 2018-04-13
Answer
According to the expression profile of left uterine tube, NAXE is highly expressed in left uterine tube. So, it is likely that anti-AIBP antibody A10537 will work for IF with left uterine tube.
Boster Scientific Support
Answered: 2018-04-13
Question
Will A10537 anti-AIBP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Patel
Verified customer
Asked: 2016-02-17
Answer
As indicated on the product datasheet, A10537 anti-AIBP antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-02-17
Question
We are currently using anti-AIBP antibody A10537 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
T. Krishna
Verified customer
Asked: 2014-09-02
Answer
The anti-AIBP antibody (A10537) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-02
