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Product Info Summary
| SKU: | A02166-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human POR DyLight® 488 conjugated Antibody
View all POR/Cytochrome P450 Reductase Antibodies
SKU/Catalog Number
A02166-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human POR DyLight® 488 conjugated Antibody catalog # A02166-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human POR DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02166-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human POR , different from the related mouse and rat sequences by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02166-Dyl488 is reactive to POR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
76.69kDa
Background of POR/Cytochrome P450 Reductase
POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02166-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: A549 cell
Validation Images & Assay Conditions
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1. Flow Cytometry analysis of A549 cells using anti-Human POR antibody (A02166-Dyl488).
Overlay histogram showing A549 cells stained with A02166-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human POR Antibody (A02166-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For POR (Source: Uniprot.org, NCBI)
Gene Name
POR
Full Name
NADPH--cytochrome P450 reductase
Weight
76.69kDa
Superfamily
NADPH--cytochrome P450 reductase family
Alternative Names
NADPH--cytochrome P450 reductase POR CPR, CYPOR, P450R cytochrome p450 oxidoreductase NADPH--cytochrome P450 reductase|NADPH--hemoprotein reductase|NADPH-dependent cytochrome P450 reductase|P450 (cytochrome) oxidoreductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POR, check out the POR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human POR DyLight® 488 conjugated Antibody (A02166-Dyl488)
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Customer Q&As
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4 Customer Q&As for Anti-Human POR DyLight® 488 conjugated Antibody
Question
Can you help my question with product A02166-Dyl488, anti-Human POR DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02166-Dyl488 anti-Human POR DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
I was wanting to use your anti-Human POR DyLight® 488 conjugated antibody for Flow Cytometry for human right adrenal gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right adrenal gland identification?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
You can see on the product datasheet, A02166-Dyl488 anti-Human POR DyLight® 488 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human right adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
Here is the WB image, lot number and protocol we used for right adrenal gland using anti-Human POR DyLight® 488 conjugated antibody A02166-Dyl488. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-05
Question
I see that the anti-Human POR DyLight® 488 conjugated antibody A02166-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
G. Zhao
Verified customer
Asked: 2015-11-09
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-11-09
