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Product Info Summary
| SKU: | PA2269 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Insulin receptor substrate 1 IRS1 Antibody Picoband®
SKU/Catalog Number
PA2269
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insulin receptor substrate 1 IRS1 Antibody catalog # PA2269. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin receptor substrate 1 IRS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2269)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse IRS1, identical to the related rat sequence, and different from the related human sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2269 is reactive to Irs1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
132 kDa
Calculated molecular weight
130723 MW
Background of IRS1
Insulin receptor substrate 1 (IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also plays important biological function for both metabolic and mitogenic (growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2269 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Whole Cell, A549 Whole Cell, 293T Whole Cell, MCF-7 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-IRS1 antibody, PA2269, All Western blotting
All lanes: Anti-IRS1(PA2269) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 132KD
Observed bind size: 132KD
Protein Target Info & Infographic
Gene/Protein Information For Irs1 (Source: Uniprot.org, NCBI)
Gene Name
Irs1
Full Name
Insulin receptor substrate 1
Weight
130723 MW
Alternative Names
Insulin receptor substrate 1;IRS-1;Irs1;Irs-1; IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Irs1, check out the Irs1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Irs1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin receptor substrate 1 IRS1 Antibody Picoband® (PA2269)
Hello CJ!
PA2269 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births
Mi L, Chen Y, Zheng X, Li Y, Zhang Q, Mo D, Yang G. J Cell Biochem. 2015 Jul;116(7):1195-204. Doi: 10.1002/Jcb.25065. Microrna-139-5P Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch And Irs1/Pi3K/Akt Insulin Signaling Pathways.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Insulin receptor substrate 1 IRS1 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for eye using anti-IRS1 antibody PA2269. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-06
Question
Do you have a BSA free version of anti-IRS1 antibody PA2269 available?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IRS1 antibody PA2269 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-04
Question
Will anti-IRS1 antibody PA2269 work on pig WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Our lab technicians have not validated anti-IRS1 antibody PA2269 on pig. You can run a BLAST between pig and the immunogen sequence of anti-IRS1 antibody PA2269 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-01
Question
I see that the anti-IRS1 antibody PA2269 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-03
Question
Will anti-IRS1 antibody PA2269 work for WB with eye?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to the expression profile of eye, IRS1 is highly expressed in eye. So, it is likely that anti-IRS1 antibody PA2269 will work for WB with eye.
Boster Scientific Support
Answered: 2020-02-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-IRS1 antibody PA2269. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
Is a blocking peptide available for product anti-IRS1 antibody (PA2269)?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do provide the blocking peptide for product anti-IRS1 antibody (PA2269). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-21
Question
I am looking for to test anti-IRS1 antibody PA2269 on human eye for research purposes, then I may be interested in using anti-IRS1 antibody PA2269 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The products we sell, including anti-IRS1 antibody PA2269, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-06
Question
We are currently using anti-IRS1 antibody PA2269 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
E. Singh
Verified customer
Asked: 2019-11-05
Answer
The anti-IRS1 antibody (PA2269) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
Does PA2269 anti-IRS1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
It shows on the product datasheet, PA2269 anti-IRS1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-27
Question
Can you help my question with product PA2269, anti-IRS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2269 anti-IRS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-06
Question
Is this PA2269 anti-IRS1 antibody reactive to the isotypes of IRS1?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The immunogen of PA2269 anti-IRS1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse IRS1(106-122aa WYQALLQLHNRAKAHHD), identical to the related rat sequence, and different from the related human sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-07
Question
We are interested in using your anti-IRS1 antibody for positive regulation of fatty acid beta-oxidation studies. Has this antibody been tested with western blotting on a549 whole cell lysate? We would like to see some validation images before ordering.
R. Zhao
Verified customer
Asked: 2018-10-09
Answer
Thanks for your inquiry. This PA2269 anti-IRS1 antibody is tested on hela whole cell lysate, a549 whole cell lysate, 293t whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-10-09
Question
We have observed staining in rat skeletal muscle. Any tips? Is anti-IRS1 antibody supposed to stain skeletal muscle positively?
M. Jones
Verified customer
Asked: 2018-05-23
Answer
From what I have seen in literature skeletal muscle does express IRS1. From what I have seen in Uniprot.org, IRS1 is expressed in endometrium, skeletal muscle, eye, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have IRS1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 8513971
Boster Scientific Support
Answered: 2018-05-23
Question
I was wanting to use your anti-IRS1 antibody for WB for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
It shows on the product datasheet, PA2269 anti-IRS1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-08
