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Product Info Summary
| SKU: | M02228 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PDE4 (PDE4B) Mouse Monoclonal Antibody [Clone ID: OTI1D12]
SKU/Catalog Number
M02228
Size
100 µl
Description
Boster Bio PDE4B mouse monoclonal antibody, clone OTI1D12 (formerly 1D12). Catalog# M02228. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PDE4 (PDE4B) Mouse Monoclonal Antibody [Clone ID: OTI1D12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02228)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1D12
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PDE4B (NP_002591) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02228 is reactive to PDE4B in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
83.343kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02228 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-PDE4B mouse monoclonal antibody (M02228) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B.
Protein Target Info & Infographic
Gene/Protein Information For PDE4B (Source: Uniprot.org, NCBI)
Gene Name
PDE4B
Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4B
Weight
83.343kDa
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
cAMP-specific (phosphodiesterase E4 dunce homolog; cAMP-specific 3'-5'-cyclic phosphodiesterase 4B; cAMP-specific phosphodiesterase-4 B isoform; DKFZp686F2182; Drosophila); dunce-like phosphodiesterase E4; EC 3.1.4; EC 3.1.4.17; MGC126529; PDE32; PDEIVB; phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E4); phosphodiesterase 4B, cAMP-specific (dunce; phosphodiesterase 4B, cAMP-specific; phosphodiesterase E4 dunce homolog PDE4B DPDE4, PDEIVB phosphodiesterase 4B cAMP-specific 3,5-cyclic phosphodiesterase 4B|dunce-like phosphodiesterase E4|phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE4B, check out the PDE4B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDE4 (PDE4B) Mouse Monoclonal Antibody [Clone ID: OTI1D12] (M02228)
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