DLAT Antibodies

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT)

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. .

Gene Information

Human
Gene Name:	DLAT
Uniprot:	P10515
Entrez:	1737

Family

Belongs to:
2-oxoacid dehydrogenase family

Alternative Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis; dihydrolipoamide S-acetyltransferase; DLTA; E2 component of pyruvate dehydrogenase complex; EC 2.3.1; M2 antigen complex 70 kDa subunit; PBC; PDC-E2EC 2.3.1.12; PDCE2mitochondrial

Molecular Weight

Mass (kDA):
68.997 kDA

Genomic Context

Human
Location:	11q23.1
Sequence:	11; NC_000011.10 (112025408..112064404)

Subcellular Localizations

Mitochondrion matrix.

Diseases

• Liver Cirrhosis
• Biliary Cirrhosis
• Primary Biliary Cirrhosis
• Autoimmune Reaction
• Autoimmune Diseases
• Malignant Neoplasms
• Cholangitis
• Lymphoma
• Neoplasms
• Acidosis
• Acidosis, Lactic
• Inflammation

• Pyruvate Dehydrogenase Complex Deficiency Disease
• Autoimmunity
• Liver Diseases
• Ataxia
• Hepatitis
• Muscle Hypotonia
• Arthritis

Interacting Proteins

• PDHB
• PDK3
• SIRT4

Pathways

• Cell Proliferation
• Pathogenesis
• Transport
• Cell Death
• Protein Phosphorylation
• Tricarboxylic Acid Cycle
• Electron Transport
• Mitosis
• Cytokine Secretion
• Virulence
• Tropism
• Localization
• Cytokine Production

• Gliogenesis
• Protein Folding
• Regulation Of Cell Proliferation
• Feeding Behavior
• Cell Recognition
• Granuloma Formation
• Excretion

PTMs

• Phosphorylation

Research Areas

• Core ESC-Like Genes
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DLAT

Boster Bio - Best Uses Of The DLAT Marker

Boster Bio founder Steven Boster created his first product in 1993. He quickly became known as "the man who brought science in the toilet" after he had developed hundreds of primary antibody products. Boster was China's largest catalog antibody company by end of the 1990s. Boster uses trade secrets to create high-sensitivity ELISA kit. Here are a few of his most popular products.

In situ hybridization detection kit

The Gal-3 in situ hybridization detection kit, manufactured by Wuhan Boster Bio-engineering Co., Ltd., PR China, is used for in situ hybridization. The samples were washed with x2 salt citrate at 37°C. After blocking for 30 minutes, biotinylated mouse anti-digoxin (Biotinylated Mouse Anti-digoxin) was added. The samples were incubated at 37degC over 1 h, washed again, and the colours were developed using DAB. Positive and negative control samples were used as controls. Light microscopy was employed to observe positive and negative signals.

Primary antibodies with high affinity

The discovery of high-affinity primary antibodies for GPC3 in HCC cells has significant therapeutic implications, since these antibodies can recognize a broad variety of cell surface-associated proteins. These antibodies also detect low expression of GPC3 in ovarian clear cell cancer and melanoma cell lines. The development of high-affinity antibodies to GPC3 is a broad approach to identify therapeutic antibodies against cancer.

First, prepare an appropriate solution comprising 50-100ul primary antibody and DLAT marker. The solution should be kept from light and placed in a plastic container. It is recommended to store it at 4 degrees Celsius. The second step is spinning the slides. After spinning, add PBS and let them sit for 30 minutes. Replace the buffer after this period, and then store them in plastic holders so that they are safe from the risk of light exposure.

There are a variety of issues that arise when detecting specific antigens. It can be difficult to tell the difference between two antigens, especially if they belong to the same species. Fortunately, it is possible to identify subtype-specific secondary antibodies that avoid the double-staining issue. Another alternative is to label the primary antibody with the use of a fluorescent dye. The fluorescent dye can be purchased from Molecular Probes. It removes one round of signal amplification. This method is particularly useful for proteins that are abundant in tissues.

The process of making the fusion proteins is more complicated than purchasing peptides. However this method is more precise in producing effective antibodies. It is important that you choose a manufacturer that offers enough fusion proteins to test the affinity purification process as well as depletion and depletion. The DLAT marker is a biomarker with high specificity, is able to distinguish high-affinity primary antibodies and low-affinity secondary antibody.

The GPC3 protein has a native form and is found on the cell surface in both the native and heparan sulfate forms. Utilizing DLAT as a marker for GPC3 mAbs will aid in the development of new treatments. It is a powerful tool for researching diseases such as Alzheimer's and cancer. It can also be used to identify antigens in the blood.

The process of determining whether the antigen present is in fact present must include fixation. Based on the type and the severity of the antibody, the conditions for fixing may differ. Fixation conditions differ based on the type of antibody as well as the tissue of interest. Other options for fixing include chemical permeabilization and collagenase treatment. The final fixation method will differ based on the antigen that is targeted and its accessibility.