Product Info Summary
SKU: | A00441 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Patched (A2) PTCH1 Antibody
View all Patched 1/PTCH Antibodies
SKU/Catalog Number
A00441
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Patched (A2) PTCH1 Antibody catalog # A00441. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Patched (A2) PTCH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00441)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Patched.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00441 is reactive to PTCH1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
160545 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00441 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of PTCH1 using Anti-Patched (A2) PTCH1 Antibody (A00441).
Immunohistochemistry (IHC) analyzes of Patched (A2) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Patched (A2) PTCH1 Antibody A00441
Western blot (WB) analysis of Patched (A2) polyclonal antibody at 1:500 dilutionLane1:HCT116 whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Lane5:The Testis tissue lysate of Mouse(40ug)
Lane6:The Testis tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PTCH1 (Source: Uniprot.org, NCBI)
Gene Name
PTCH1
Full Name
Protein patched homolog 1
Weight
160545 MW
Superfamily
patched family
Alternative Names
Protein patched homolog 1;PTC;PTC1;PTCH1;PTCH; PTCH1 BCNS, NBCCS, PTC, PTC1, PTCH patched 1 protein patched homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTCH1, check out the PTCH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTCH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Patched (A2) PTCH1 Antibody (A00441)
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No publications found for A00441
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