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Product Info Summary
| SKU: | A00751-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-DDX3 DDX3X Antibody
SKU/Catalog Number
A00751-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DDX3 DDX3X Antibody (Catalog # A00751-2). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
DDX3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DDX3 DDX3X Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00751-2)
Host
Rabbit
Contents
DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3. The immunogen is located within amino acids 110 - 160 of DDX3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DDX3 antibody will detect both DDX3 and DBY.
Reactive Species
A00751-2 is reactive to DDX3X in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
73243 MW
Background of DDX3
DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00751-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.
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Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DDX3X (Source: Uniprot.org, NCBI)
Gene Name
DDX3X
Full Name
ATP-dependent RNA helicase DDX3X
Weight
73243 MW
Superfamily
DEAD box helicase family
Alternative Names
DBX, DDX3, HLP2, DDX14, DBX, ATP-dependent RNA helicase DDX3X, DEAD box protein 3, X-chromosomal DDX3X CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB DEAD-box helicase 3 X-linked ATP-dependent RNA helicase DDX3X|DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked|DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|DEAD box protein 3, X-chromosomal|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX3X, check out the DDX3X Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX3X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX3 DDX3X Antibody (A00751-2)
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