Human GAS1 ELISA Kit PicoKine®

GAS1 ELISA kit for Human

Human GAS1 ELISA Kit PicoKine® (96 Tests). Quantitate Human GAS1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2044
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human GAS1 ELISA Kit PicoKine®

View all GAS1 ELISA kits

SKU/Catalog Number

EK2044

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GAS1 ELISA Kit PicoKine® (96 Tests). Quantitate Human GAS1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GAS1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2044)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: L40-S318

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2044 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2044 is reactive to GAS1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2044 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GAS1

Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 9q21.33. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

Kit Components

Catalog Number Description Quantity
EK2044-CAP Anti-Human GAS1 Pre-coated 96-well strip microplate 1
EK2044-ST Human GAS1 Standard 2 vials, 2 ng/tube
EK2044-DA Human GAS1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0560.1380.2380.3650.6571.1511.6482.230

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect GAS1, Dilution ratio of 1:1, concentration in serum and plasma is around 60 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GAS1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5522884560239884
Standard deviation2.3114.3638.033.1815.3063.65
CV (%)4.2%6.3%4.5%5.3%6.4%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GAS1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155535052521.803.4%
Sample 222826122826424517.287.0%
Sample 384578883884582923.842.8%
*number of samples for each test n=16.

Gene/Protein Information For GAS1 (Source: Uniprot.Org, NCBI)

Gene Name

GAS1

Full Name

Growth arrest-specific protein 1

Weight

35.693kDa

Alternative Names

Growth arrest-specific protein 1; GAS-1; GAS1 GAS1 growth arrest specific 1 growth arrest-specific protein 1|GAS-1|Growth arrest-specific gene-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GAS1, check out the GAS1 Infographic

GAS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GAS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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5 Customer Q&As for Human GAS1 ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-03-03

Question

Q: What is the optimal O.D. value for GAS1 ELISA kit? I performed your GAS1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain GAS1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GAS1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GAS1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-08-27

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-03-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-03-22

Question

Q: is it okay to use citrate plasma as samples in Human GAS1 Picokine® ELISA Kit (Catalog # EK2044)?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of GAS1 causing disruption of its protein structure. GAS1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the GAS1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-11-23

Question

Q: how can I thaw whole blood sample for GAS1 ELISA after freezing?

D. Dhawan

Verified customer

Asked: 2016-06-18

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GAS1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-06-18

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