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Product Info Summary
| SKU: | M02445 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PABPN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02445
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PABPN1 Rabbit Monoclonal Antibody catalog # M02445. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PABPN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02445)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAHI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PABPN1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02445 is reactive to PABPN1 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
53 kDa
Calculated molecular weight
32749 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02445 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of PABPN1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PABPN1 (Source: Uniprot.org, NCBI)
Gene Name
PABPN1
Full Name
Polyadenylate-binding protein 2
Weight
32749 MW
Alternative Names
Polyadenylate-binding protein 2;PABP-2;Poly (A)-binding protein 2;Nuclear poly (A)-binding protein 1;Poly (A)-binding protein II;PABII;Polyadenylate-binding nuclear protein 1;PABPN1;PAB2, PABP2; PABPN1 OPMD, PAB2, PABII, PABP-2, PABP2 poly(A) binding protein nuclear 1 polyadenylate-binding protein 2|poly(A) binding protein 2|poly(A) binding protein II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PABPN1, check out the PABPN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PABPN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PABPN1 Rabbit Monoclonal Antibody (M02445)
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No publications found for M02445
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PABPN1 Rabbit Monoclonal Antibody
Question
We are currently using anti-PABPN1 Rabbit Monoclonal antibody M02445 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The anti-PABPN1 Rabbit Monoclonal antibody (M02445) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-13
Question
Is this M02445 anti-PABPN1 Rabbit Monoclonal antibody reactive to the isotypes of PABPN1?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
The immunogen of M02445 anti-PABPN1 Rabbit Monoclonal antibody is A synthesized peptide derived from human PABPN1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-16
Question
Would anti-PABPN1 Rabbit Monoclonal antibody M02445 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
According to the expression profile of leukemic t-cell, PABPN1 is highly expressed in leukemic t-cell. So, it is likely that anti-PABPN1 Rabbit Monoclonal antibody M02445 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-10-15
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-PABPN1 Rabbit Monoclonal antibody M02445. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-10
Question
Is a blocking peptide available for product anti-PABPN1 Rabbit Monoclonal antibody (M02445)?
G. Anderson
Verified customer
Asked: 2014-03-21
Answer
We do provide the blocking peptide for product anti-PABPN1 Rabbit Monoclonal antibody (M02445). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-21
Question
Can you help my question with product M02445, anti-PABPN1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Jones
Verified customer
Asked: 2013-01-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02445 anti-PABPN1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-07
