Anti-eIF4A1 Monoclonal Antibody

Product Name

Anti-eIF4A1 Monoclonal Antibody

View all EIF4A1 Antibodies

SKU/Catalog Number

M03922

Size

100ul

Form

Liquid

Description

Boster Bio Anti-eIF4A1 Monoclonal Antibody catalog # M03922. Tested in IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eIF4A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03922)

Host

Mouse

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

M8

Isotype

IgG

Immunogen

Synthetic Peptide

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M03922 is reactive to EIF4A1 in Human, Mouse, Rat

Applications

M03922 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46154 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-3000
IF 1:100-200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-eIF4A1 Monoclonal Antibody M03922

Western blot (WB) analysis of eIF4A1 monoclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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Figure 2. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of human-colon-cancer tissue. Anti-eIF4A1 at 1:200 (4°C

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Figure 3. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of human liver cancer tissue. Anti-eIF4A1 at 1:200 (4°C

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Figure 4. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of human liver tissue. Anti-eIF4A1 at 1:200 (4°C

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Figure 5. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of human stomach cancer tissue. Anti-eIF4A1 at 1:200 (4°C

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Figure 6. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of mouse brain tissue. Anti-eIF4A1 at 1:200 (4°C

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Figure 7. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of mouse colon tissue. Anti-eIF4A1 at 1:200 (4°C

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Figure 8. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of mouse kidney tissue. M03922 was diluted at 1:200 (4°C

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Figure 9. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of rat lung tissue. M03922 was diluted at 1:200 (4°C

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Figure 10. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of rat spinal cord tissue. M03922 was diluted at 1:200 (4°C

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Figure 11. Immunohistochemistry validation of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunohistochemical analysis of rat spleen tissue. M03922 was diluted at 1:200 (4°C

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Figure 12. Immunofluorescent staining data of EIF4A1 using Anti-eIF4A1 Monoclonal Antibody (M03922).

Immunofluorescence (IF) analysis of HeLa with antibody (Left) and DAPI (Right) diluted at 1:100.

Gene/Protein Information For EIF4A1 (Source: Uniprot.org, NCBI)

Alternative Names

ATP-dependent RNA helicase eIF4A-1; DDX2Aeukaryotic initiation factor 4A-I; EC 3.6.1; EC 3.6.4.13; EIF4A; EIF-4A; eIF-4A-I; eIF4A-I; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A1 EIF4A1 DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I eukaryotic translation initiation factor 4A1 eukaryotic initiation factor 4A-I|ATP-dependent RNA helicase eIF4A-1|eukaryotic initiation factor 4AI|eukaryotic translation initiation factor 4A

For more info on EIF4A1, check out the EIF4A1 Infographic

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In this infographic, you will see the following information for EIF4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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