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Product Info Summary
| SKU: | EK2017 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine®
View all Osteoadherin/OSAD/OMD ELISA kits
SKU/Catalog Number
EK2017
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Osteoadherin ELISA Kit PicoKine® (96 Tests). Quantitate Human OMD in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2017)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q21-E421
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2017 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2017 is reactive to OMD in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2017 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Osteoadherin/OSAD/OMD
Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 9q22.31. Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20‑30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix. Human OSAD is expressed as an even larger 110 kDa molecule in teeth. OSAD contains eight sulfated tyrosine residues and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C‑terminal acidic domain. OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2017-CAP | Anti-Human OMD Pre-coated 96-well strip microplate | 1 |
| EK2017-ST | Human OMD Standard | 2 vials, 10 ng/tube |
| EK2017-DA | Human OMD Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.030 | 0.085 | 0.138 | 0.229 | 0.423 | 0.806 | 1.391 | 2.271 |
Data Example Images
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Human Osteoadherin PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Osteoadherin/OSAD/OMD, Dilution ratio of 1:10, concentration in serum and plasma is 3-6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 178 | 606 | 1031 | 175 | 567 | 1093 |
| Standard deviation | 9.79 | 39.99 | 75.26 | 9.62 | 38.55 | 96.18 |
| CV (%) | 5.5% | 6.6% | 7.3% | 5.5% | 6.8% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Osteoadherin/OSAD/OMD in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 178 | 162 | 182 | 159 | 170 | 9.9 | 5.8% |
| Sample 2 | 606 | 547 | 628 | 536 | 579 | 38.73 | 6.6% |
| Sample 3 | 1031 | 1040 | 1142 | 1024 | 1059 | 48.11 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For OMD (Source: Uniprot.Org, NCBI)
Gene Name
OMD
Full Name
Osteomodulin
Weight
49.492kDa
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; OMD; SLRR2C; UNQ190; PRO216 OMD OSAD, SLRR2C osteomodulin osteomodulin|KSPG osteomodulin|keratan sulfate proteoglycan osteomodulin|osteoadherin proteoglycan
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on OMD, check out the OMD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for OMD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine® (EK2017)
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Customer Q&As
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7 Customer Q&As for Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Osteoadherin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Osteoadherin antigen?
Verified Customer
Verified customer
Asked: 2019-12-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-08
Question
Q: how to analyze ELISA data? I have performed Osteoadherin level in plasma.
M. Parker
Verified customer
Asked: 2018-08-31
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-08-04
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-08-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-07-25
Question
Q: which procedure should I follow in order to thaw whole blood sample for Osteoadherin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Osteoadherin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-02-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-02-15
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-02-15
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-11-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-11-03
