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Product Info Summary
| SKU: | A01058-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-p90RSK (Ab-348) RPS6KA1 Antibody
SKU/Catalog Number
A01058-3
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-p90RSK (Ab-348) RPS6KA1 Antibody (Catalog # A01058-3). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p90RSK (Ab-348) RPS6KA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01058-3)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.346~350 (S-R-T-P-R) derived from Mouse p90RSK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01058-3 is reactive to RPS6KA1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
126870 MW
Background of RSK1
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583.
Andrew D, et al. Biochem J. 2006 February 1; 393(Pt 3): 715
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01058-3 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RPS6KA1 using anti-RPS6KA1 antibody (A01058-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA1 antigen affinity purified polyclonal antibody (Catalog # A01058-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RPS6KA1.
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Immunofluorescence staining of methanol-fixed Hela cells using p90RSK(Ab-348) Antibody #A01058-3.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KA1 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA1
Full Name
Ribosomal protein S6 kinase alpha-1
Weight
126870 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase LATS1;2.7.11.1;Large tumor suppressor homolog 1;WARTS protein kinase;h-warts;LATS1 ;WARTS ; RPS6KA1 HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk ribosomal protein S6 kinase A1 ribosomal protein S6 kinase alpha-1|90 kDa ribosomal protein S6 kinase 1|MAP kinase-activated protein kinase 1a|MAPK-activated protein kinase 1a|MAPKAP kinase 1a|MAPKAPK-1a|RSK-1|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|p90RSK1|p90S6K|ribosomal S6 kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA1, check out the RPS6KA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p90RSK (Ab-348) RPS6KA1 Antibody (A01058-3)
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