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Product Info Summary
| SKU: | PA2052 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RSK1 p90/RPS6KA1 Antibody Picoband®
SKU/Catalog Number
PA2052
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RSK1 p90/RPS6KA1 Antibody catalog # PA2052. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RSK1 p90/RPS6KA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2052)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RSK1 p90, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2052 is reactive to RPS6KA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated molecular weight
82723 MW
Background of RSK1
RPS6KA1 (Ribosomal Protein S6 Kinase, 90-KD, 1), also called RSK1, is an enzyme that in humans is encoded by the RPS6KA1 gene. The RSK (ribosomal S6 kinase) family comprises growth factor-regulated serine/threonine kinases, known also as p90 (rsk). Homologs of RSK exist in several species. By analysis of somatic cell hybrids, Moller et al. (1994) mapped the RPS6KA1 gene to chromosome 3.Bonni et al. (1999) characterized the mechanism by which the RAS-MAPK signaling pathway mediates growth factor-dependent cell survival. Inoue et al. (2007) demonstrated that p90rsk, the kinase immediately downstream from Mos-MAPK, directly targets Erp1 for CSF arrest in Xenopus oocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2052 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: MCF-7 Cell, HELA Cell, K562 Cell, JURKAT Cell, SW620 Cell, RAJI Cell, A431 Whole Cell, MCF-7 Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-RSK1 p90 antibody, PA2052, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: K562 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: SW620 Cell Lysate
Lane 6: RAJI Cell Lysate
Click image to see more details
Anti-RSK1 p90 antibody, PA2052, All Western blotting
All lanes: Anti-RPS6KA1 (PA2052) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
Protein Target Info & Infographic
Gene/Protein Information For RPS6KA1 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA1
Full Name
Ribosomal protein S6 kinase alpha-1
Weight
82723 MW
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase alpha-1;S6K-alpha-1;2.7.11.1;90 kDa ribosomal protein S6 kinase 1;p90-RSK 1;p90RSK1;p90S6K;MAP kinase-activated protein kinase 1a;MAPK-activated protein kinase 1a;MAPKAP kinase 1a;MAPKAPK-1a;Ribosomal S6 kinase 1;RSK-1;RPS6KA1;MAPKAPK1A, RSK1; RPS6KA1 HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk ribosomal protein S6 kinase A1 ribosomal protein S6 kinase alpha-1|90 kDa ribosomal protein S6 kinase 1|MAP kinase-activated protein kinase 1a|MAPK-activated protein kinase 1a|MAPKAP kinase 1a|MAPKAPK-1a|RSK-1|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|p90RSK1|p90S6K|ribosomal S6 kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA1, check out the RPS6KA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RSK1 p90/RPS6KA1 Antibody Picoband® (PA2052)
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No publications found for PA2052
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Customer Q&As
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3 Customer Q&As for Anti-RSK1 p90/RPS6KA1 Antibody Picoband®
Question
We are currently using anti-RSK1 p90/RPS6KA1 antibody PA2052 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The anti-RSK1 p90/RPS6KA1 antibody (PA2052) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
I have a question about product PA2052, anti-RSK1 p90/RPS6KA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2052 anti-RSK1 p90/RPS6KA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-13
Question
I see that the anti-RSK1 p90/RPS6KA1 antibody PA2052 works with WB, what is the protocol used to produce the result images on the product page?
C. Williams
Verified customer
Asked: 2013-09-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-25
