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Product Info Summary
| SKU: | PA2053 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-p53R2/RRM2B Antibody Picoband®
SKU/Catalog Number
PA2053
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p53R2/RRM2B Antibody catalog # PA2053. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p53R2/RRM2B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2053)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human p53R2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2053 is reactive to RRM2B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated molecular weight
40737 MW
Background of p53R2
RRM2B (Ribonucleotide Reductase, M2 B), also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al. (2000) mapped the p53R2 gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al. (2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2053 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Thymus Tissue, MCF-7 Cell, A431 Cell, HELA Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-p53R2 antibody, PA2053, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: HELA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For RRM2B (Source: Uniprot.org, NCBI)
Gene Name
RRM2B
Full Name
Ribonucleoside-diphosphate reductase subunit M2 B
Weight
40737 MW
Superfamily
ribonucleoside diphosphate reductase small chain family
Alternative Names
Ribonucleoside-diphosphate reductase subunit M2 B;1.17.4.1;TP53-inducible ribonucleotide reductase M2 B;p53-inducible ribonucleotide reductase small subunit 2-like protein;p53R2;RRM2B;P53R2; RRM2B MTDPS8A, MTDPS8B, P53R2 ribonucleotide reductase regulatory TP53 inducible subunit M2B ribonucleoside-diphosphate reductase subunit M2 B|TP53-inducible ribonucleotide reductase M2 B|p53-inducible ribonucleotide reductase small subunit 2 homolog|p53-inducible ribonucleotide reductase small subunit 2 short form beta|p53-inducible ribonucleotide reductase small subunit 2-like protein|ribonucleotide reductase M2 B (TP53 inducible)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRM2B, check out the RRM2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRM2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p53R2/RRM2B Antibody Picoband® (PA2053)
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No publications found for PA2053
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Customer Q&As
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1 Customer Q&As for Anti-p53R2/RRM2B Antibody Picoband®
Question
We are currently using anti-p53R2/RRM2B antibody PA2053 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The anti-p53R2/RRM2B antibody (PA2053) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-07
