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Product Info Summary
| SKU: | A01576 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein phosphatase 1D PPM1D Antibody
SKU/Catalog Number
A01576
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein phosphatase 1D PPM1D Antibody (Catalog # A01576). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PPM1D antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein phosphatase 1D PPM1D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01576)
Host
Rabbit
Contents
PPM1D antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D. The immunogen is located within the last 50 amino acids of PPM1D.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.
Reactive Species
A01576 is reactive to PPM1D in Human, Mouse, Rat
Applications
A01576 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
45681 MW
Background of PPM1D
Wild-type p53 induced phosphatase 1 (WIP1) / protein phosphatase magnesium-dependent 1 delta (PPM1D), a protein identified in the p53 DNA response pathway, is a member of the PP2C family of serine/threonine protein phosphatases which selectively inactivates p38 MAPK and dephosphorylates the ATM/ATR targets, Chk1 and p53 (1,2). Studies have shown that it is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (2,3). PPM1D deletion results in a reduction of T and B cell function and compromised cell division, rendering cells resistant to becoming cancerous and slowing tumor development (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 μg/ml.
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Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 μg/ml.
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Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PPM1D (Source: Uniprot.org, NCBI)
Gene Name
PPM1D
Full Name
Protein phosphatase 1D
Weight
45681 MW
Superfamily
PP2C family
Alternative Names
EC 3.1.3.16; p53-induced protein phosphatase 1; PP2C-delta; PPM1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase 1D; protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; protein phosphatase, Mg2+/Mn2+ dependent, 1D; wild-type p53-induced phosphatase 1; Wip1; WIP1protein phosphatase Wip1 PPM1D IDDGIP, JDVS, PP2C-DELTA, WIP1 protein phosphatase, Mg2+/Mn2+ dependent 1D protein phosphatase 1D|protein phosphatase Wip1|wild-type p53-induced phosphatase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPM1D, check out the PPM1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPM1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein phosphatase 1D PPM1D Antibody (A01576)
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