Product Info Summary
SKU: | A07813 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ACSL6 Antibody
SKU/Catalog Number
A07813
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ACSL6 Antibody (Catalog # A07813). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACSL6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07813)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ACSL6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07813 is reactive to ACSL6 in Human, Mouse
Reconstitution
Calculated molecular weight
77752 MW
Background of ACSL6
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.
Malhotra K.T., Biochem. J. 344:135-143(1999).
Yagasaki F., Genes Chromosomes Cancer 26:192-202(1999).
Nagase T., DNA Res. 5:355-364(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07813 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COLO cells and Jurkat cells, using ACSL6 antibody A07813.
Protein Target Info & Infographic
Gene/Protein Information For ACSL6 (Source: Uniprot.org, NCBI)
Gene Name
ACSL6
Full Name
Long-chain-fatty-acid--CoA ligase 6
Weight
77752 MW
Superfamily
ATP-dependent AMP-binding enzyme family
Alternative Names
Long-chain-fatty-acid--CoA ligase 6;6.2.1.3;Long-chain acyl-CoA synthetase 6;LACS 6;ACSL6;ACS2, FACL6, KIAA0837, LACS5; ACSL6 ACS2, FACL6, LACS 6, LACS2, LACS5 acyl-CoA synthetase long chain family member 6 long-chain-fatty-acid--CoA ligase 6|arachidonate--CoA ligase|fatty-acid-Coenzyme A ligase, long-chain 6|long fatty acyl-CoA synthetase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACSL6, check out the ACSL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACSL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACSL6 Antibody (A07813)
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No publications found for A07813
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Customer Q&As
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5 Customer Q&As for Anti-ACSL6 Antibody
Question
I was wanting to use your anti-ACSL6 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
As indicated on the product datasheet, A07813 anti-ACSL6 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-ACSL6 antibody A07813. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
Can you help my question with product A07813, anti-ACSL6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07813 anti-ACSL6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-21
Question
Does anti-ACSL6 antibody A07813 work on horse WB with brain?
E. Carter
Verified customer
Asked: 2015-02-18
Answer
Our lab technicians have not validated anti-ACSL6 antibody A07813 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ACSL6 antibody A07813 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-18
Question
Is this A07813 anti-ACSL6 antibody reactive to the isotypes of ACSL6?
S. Evans
Verified customer
Asked: 2015-02-13
Answer
The immunogen of A07813 anti-ACSL6 antibody is Synthesized peptide derived from internal of human ACSL6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-13