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Product Info Summary
| SKU: | A02118 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p130 Rb2 RBL2 Antibody
SKU/Catalog Number
A02118
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-p130 Rb2 RBL2 Antibody (Catalog # A02118). Tested in IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-p130 Rb2 RBL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02118)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02118 is reactive to RBL2 in Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
128367 MW
Background of p130
Retinoblastoma-like protein 2 (Rb2) is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. p130 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 'Lys-20' trimethylation and probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. It is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E as well as binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Rb2 may act as a tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02118 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:200 - 1:1,000
IP: 1:100
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Western blot analysis of p130 Rb2 expression in HEK 293 pcDNA3 whole cell lysates (lane 1), HEK 293 pcDNA3-Rb2wt whole cell lysates (lane 2) and HEK 293 pcDNA3-Rb2-PM19 whole cell lysates (lane 3). p130 Rb2 at 130KD was detected using rabbit anti-p130 Rb2 polyclonal antibody (Catalog # A02118) at 1:250. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
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p130 Rb2was detected in immunohistochemical staining of mouse tissue using rabbit anti-p130 Rb2 polyclonal antibody (Catalog # A02118) at 1:500. The staining shows the location of p130 Rb2 in developing mouse tissue. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Protein Target Info & Infographic
Gene/Protein Information For RBL2 (Source: Uniprot.org, NCBI)
Gene Name
RBL2
Full Name
Retinoblastoma-like protein 2
Weight
128367 MW
Superfamily
retinoblastoma protein (RB) family
Alternative Names
p130, Rb2, RBR 2, RBR2, Retinoblastoma like 2, Retinoblastoma like protein 2, Retinoblastoma Related Gene RBL2 P130, Rb2 RB transcriptional corepressor like 2 retinoblastoma-like protein 2|130 kDa retinoblastoma-associated protein|PRB2|RBR-2|retinoblastoma-like 2|retinoblastoma-related protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBL2, check out the RBL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p130 Rb2 RBL2 Antibody (A02118)
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