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Product Info Summary
| SKU: | A05802 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-DNAJB11/Erdj3 Antibody
SKU/Catalog Number
A05802
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DNAJB11/Erdj3 Antibody (Catalog # A05802). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNAJB11/Erdj3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05802)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human DNAJB11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05802 is reactive to DNAJB11 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
40514 MW
Background of DNAJB11
Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
Ohtsuka K., Cell Stress Chaperones 5:98-112(2000).
Yu M., J. Biol. Chem. 275:24984-24992(2000).
Bies C., Biol. Chem. 385:389-395(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05802 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, HUVEC cells, COLO cells and MCF-7 cells, using DNAJB11 antibody A05802.
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using DNAJB11 antibody A05802.
Protein Target Info & Infographic
Gene/Protein Information For DNAJB11 (Source: Uniprot.org, NCBI)
Gene Name
DNAJB11
Full Name
DnaJ homolog subfamily B member 11
Weight
40514 MW
Alternative Names
DnaJ homolog subfamily B member 11;APOBEC1-binding protein 2;ABBP-2;DnaJ protein homolog 9;ER-associated DNAJ;ER-associated Hsp40 co-chaperone;Endoplasmic reticulum DNA J domain-containing protein 3;ER-resident protein ERdj3;ERdj3;ERj3p;HEDJ;Human DnaJ protein 9;hDj-9;PWP1-interacting protein 4;DNAJB11;EDJ, ERJ3, HDJ9;PSEC0121, UNQ537/PRO1080; DNAJB11 ABBP-2, ABBP2, DJ9, Dj-9, EDJ, ERdj3, ERj3, ERj3p, PKD6, PRO1080, UNQ537 DnaJ heat shock protein family (Hsp40) member B11 dnaJ homolog subfamily B member 11|APOBEC1-binding protein 2|DnaJ (Hsp40) homolog, subfamily B, member 11|DnaJ protein 9|ER-associated DNAJ protein 3|ER-associated Hsp40 co-chaperone|ER-resident protein ERdj3|PWP1-interacting protein 4|endoplasmic reticulum DNA J domain-containing protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNAJB11, check out the DNAJB11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNAJB11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNAJB11/Erdj3 Antibody (A05802)
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No publications found for A05802
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Customer Q&As
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4 Customer Q&As for Anti-DNAJB11/Erdj3 Antibody
Question
I was wanting to use your anti-DNAJB11/Erdj3 antibody for IHC for rat embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
As indicated on the product datasheet, A05802 anti-DNAJB11/Erdj3 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-20
Question
Would anti-DNAJB11/Erdj3 antibody A05802 work for IHC with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
According to the expression profile of embryonic kidney, DNAJB11 is highly expressed in embryonic kidney. So, it is likely that anti-DNAJB11/Erdj3 antibody A05802 will work for IHC with embryonic kidney.
Boster Scientific Support
Answered: 2019-12-20
Question
We want to test anti-DNAJB11/Erdj3 antibody A05802 on rat embryonic kidney for research purposes, then I may be interested in using anti-DNAJB11/Erdj3 antibody A05802 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The products we sell, including anti-DNAJB11/Erdj3 antibody A05802, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-11
Question
Does A05802 anti-DNAJB11/Erdj3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
As indicated on the product datasheet, A05802 anti-DNAJB11/Erdj3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-27
