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Product Info Summary
| SKU: | EK1780 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human Contactin-4 ELISA Kit PicoKine®
View all Contactin-4 ELISA kits
SKU/Catalog Number
EK1780
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Contactin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CNTN4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Contactin-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1780)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D19-S1000
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1780 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1780 is reactive to CNTN4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1780 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Contactin-4
Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 3p26.3-p26.2. This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1780-CAP | Anti-Human CNTN4 Pre-coated 96-well strip microplate | 1 |
| EK1780-ST | Human CNTN4 Standard | 2 vials, 20 ng/tube |
| EK1780-DA | Human CNTN4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.057 | 0.101 | 0.156 | 0.256 | 0.456 | 0.896 | 1.592 | 2.256 |
Data Example Images
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Human Contactin-4 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Contactin-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 343 | 1884 | 8803 | 425 | 2121 | 9479 |
| Standard deviation | 14.75 | 97.97 | 404.94 | 25.93 | 125.14 | 701.45 |
| CV (%) | 4.3% | 5.2% | 4.6% | 6.1% | 5.9% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Contactin-4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 343 | 376 | 320 | 370 | 352 | 22.38 | 6.3% |
| Sample 2 | 1884 | 1754 | 1627 | 1875 | 1785 | 104.67 | 5.8% |
| Sample 3 | 8803 | 9355 | 8126 | 9099 | 8845 | 459.16 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For CNTN4 (Source: Uniprot.Org, NCBI)
Gene Name
CNTN4
Full Name
Contactin-4
Weight
113.393kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2; CNTN4 Cntn4|Axcam, Big-2|contactin 4|contactin-4|axonal-associated cell adhesion molecule|brain-derived immunoglobulin superfamily protein 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CNTN4, check out the CNTN4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CNTN4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Contactin-4 ELISA Kit PicoKine® (EK1780)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human Contactin-4 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-02-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-11-09
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-09
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Contactin-4 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Contactin-4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-28
Question
Q: Can Contactin-4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Varma
Verified customer
Asked: 2018-05-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-05-14
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Contactin-4 using the EK1780 Human Contactin-4 Picokine® ELISA Kit?
G. Campbell
Verified customer
Asked: 2018-01-23
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-01-23
Question
Q: What is the optimal O.D. value for Contactin-4 ELISA kit? I used your Contactin-4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Contactin-4 even though the O.D. values are not very high?
R. Green
Verified customer
Asked: 2017-09-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Contactin-4 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Contactin-4 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2017-09-21
Question
Q: how do I analyze ELISA data? I have performed Contactin-4 level in plasma.
Verified Customer
Verified customer
Asked: 2017-04-17
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-04-17
Question
Q: is it okay to use EDTA plasma as samples in Human Contactin-4 Picokine® ELISA Kit (Catalog # EK1780)?
J. Rodriguez
Verified customer
Asked: 2016-12-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Contactin-4 causing degradation of its protein structure. Contactin-4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Contactin-4 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-12-30
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-07-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-07-20
Question
Q: how can I thaw whole blood sample for Contactin-4 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2015-05-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Contactin-4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2015-05-05
