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Product Info Summary
| SKU: | M05601-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Otx1/2 Monoclonal Antibody
SKU/Catalog Number
M05601-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Otx1/2 Monoclonal Antibody catalog # M05601-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Otx1/2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05601-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADBB-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Otx1/2 Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS) : 5'-TCTAATCCC-3'.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05601-1 is reactive to OTX1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
37327 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05601-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
Positive Control
WB: Y79 cell
Validation Images & Assay Conditions
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Western blot analysis of Otx1/2 expression in Y79 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For OTX1 (Source: Uniprot.org, NCBI)
Gene Name
OTX1
Full Name
Homeobox protein OTX1
Weight
37327 MW
Superfamily
paired homeobox family
Alternative Names
Homeobox protein OTX1;Orthodenticle homolog 1;OTX1; OTX1 orthodenticle homeobox 1 homeobox protein OTX1|orthodenticle homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OTX1, check out the OTX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OTX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Otx1/2 Monoclonal Antibody (M05601-1)
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No publications found for M05601-1
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Customer Q&As
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5 Customer Q&As for Anti-Otx1/2 Monoclonal Antibody
Question
Can you help my question with product M05601-1, anti-Otx1/2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05601-1 anti-Otx1/2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-24
Question
We are currently using anti-Otx1/2 Monoclonal antibody M05601-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The anti-Otx1/2 Monoclonal antibody (M05601-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
We are interested in to test anti-Otx1/2 Monoclonal antibody M05601-1 on mouse brain for research purposes, then I may be interested in using anti-Otx1/2 Monoclonal antibody M05601-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
The products we sell, including anti-Otx1/2 Monoclonal antibody M05601-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-12
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Otx1/2 Monoclonal antibody M05601-1. Please let me know if you require anything else.
A. Krishna
Verified customer
Asked: 2018-03-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-23
Question
Do you have a BSA free version of anti-Otx1/2 Monoclonal antibody M05601-1 available?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Otx1/2 Monoclonal antibody M05601-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-24
