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Product Info Summary
| SKU: | M05601 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Otx1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05601
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Otx1 Rabbit Monoclonal Antibody catalog # M05601. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Otx1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05601)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BDH-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Otx1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05601 is reactive to OTX1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
37 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05601 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Otx1 using anti-Otx1 antibody (M05601).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2:mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Otx1 antigen affinity purified monoclonal antibody (Catalog # M05601) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Otx1 at approximately 37 kDa. The expected band size for Otx1 is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For OTX1 (Source: Uniprot.org, NCBI)
Gene Name
OTX1
Full Name
Homeobox protein OTX1
Weight
37 kDa
Superfamily
paired homeobox family
Alternative Names
Homeobox protein OTX1;Orthodenticle homolog 1;OTX1; OTX1 orthodenticle homeobox 1 homeobox protein OTX1|orthodenticle homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OTX1, check out the OTX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OTX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Otx1 Rabbit Monoclonal Antibody (M05601)
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