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Product Info Summary
| SKU: | M03355 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PHGDH Mouse Monoclonal Antibody [Clone ID: OTI5C4]
SKU/Catalog Number
M03355
Size
100 µl
Description
Boster Bio PHGDH mouse monoclonal antibody, clone OTI5C4 (formerly 5C4). Catalog# M03355. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PHGDH Mouse Monoclonal Antibody [Clone ID: OTI5C4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03355)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5C4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human PHGDH (NP_006614) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03355 is reactive to PHGDH in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
56.651kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03355 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and PHGDH-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PHGDH monoclonal antibody M03355
Protein Target Info & Infographic
Gene/Protein Information For PHGDH (Source: Uniprot.org, NCBI)
Gene Name
PHGDH
Full Name
D-3-phosphoglycerate dehydrogenase
Weight
56.651kDa
Superfamily
D-isomer specific 2-hydroxyacid dehydrogenase family
Alternative Names
3,3-phosphoglycerate dehydrogenase; 3PGDH; 3-PGDH; D-3-phosphoglycerate dehydrogenase; EC 1.1.1; EC 1.1.1.95; MGC3017; PDG; PDGH3; PGAD; PGD; PGDH; PHGDH; phosphoglycerate dehydrogenase; SERA PHGDH 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDHD, SERA, PHGDH phosphoglycerate dehydrogenase D-3-phosphoglycerate dehydrogenase|2-oxoglutarate reductase|3-phosphoglycerate dehydrogenase|epididymis secretory protein Li 113|malate dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHGDH, check out the PHGDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHGDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHGDH Mouse Monoclonal Antibody [Clone ID: OTI5C4] (M03355)
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