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Product Info Summary
| SKU: | M04934-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ORP150 Rabbit Monoclonal Antibody
View all ORP150/HSP12A Antibodies
SKU/Catalog Number
M04934-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ORP150 Rabbit Monoclonal Antibody catalog # M04934-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ORP150 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04934-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
19H99
Isotype
IgG
Immunogen
A synthesized peptide derived from human ORP150
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04934-2 is reactive to HYOU1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
150 kDa
Calculated molecular weight
20532 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04934-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Positive Control
WB: human MCF-7 whole cell, human 293T whole cell, human SH-SY5Y whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ORP150 using anti-ORP150 antibody (M04934-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORP150 antigen affinity purified monoclonal antibody (Catalog # M04934-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORP150 at approximately 150 kDa. The expected band size for ORP150 is at 111 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HYOU1 (Source: Uniprot.org, NCBI)
Gene Name
HYOU1
Full Name
Hypoxia up-regulated protein 1
Weight
20532 MW
Superfamily
heat shock protein 70 family
Alternative Names
Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis;BBC3;PUMA; HYOU1 GRP-170, Grp170, HSP12A, IMD59, ORP-150, ORP150 hypoxia up-regulated 1 hypoxia up-regulated protein 1|150 kDa oxygen-regulated protein|170 kDa glucose-regulated protein|epididymis secretory sperm binding protein|oxygen regulated protein (150kD)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HYOU1, check out the HYOU1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HYOU1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ORP150 Rabbit Monoclonal Antibody (M04934-2)
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