Anti-BAG2 Antibody Picoband®

Product Name

Anti-BAG2 Antibody Picoband®

View all BAG2 Antibodies

SKU/Catalog Number

PA2099

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BAG2 Antibody catalog # PA2099. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BAG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2099)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human BAG2, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2099 is reactive to BAG2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

140-150 kDa

Calculated molecular weight

23772 MW

Background of BAG2

BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2099 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Testis Tissue, HELA Cell, A549 Cell, A431 Cell

Validation Images & Assay Conditions

Gene/Protein Information For BAG2 (Source: Uniprot.org, NCBI)

Alternative Names

BAG family molecular chaperone regulator 2;BAG-2;Bcl-2-associated athanogene 2;BAG2; BAG2 BAG-2, dJ417I1.2 BAG cochaperone 2 BAG family molecular chaperone regulator 2|BCL2 associated athanogene 2|bcl-2-associated athanogene 2|dJ417I1.2 (BAG-family molecular chaperone regulator 2)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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