Anti-Oct4/POU5F1 Antibody Picoband™

Product Name

Anti-Oct4/POU5F1 Antibody Picoband™

View all POU5F1 Antibodies

SKU/Catalog Number

A00174-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Oct4/POU5F1 Antibody Picoband™ catalog # A00174-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Oct4/POU5F1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00174-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Oct4/POU5F1, which shares 94.1% amino acid (aa) sequence identity with mouse POU5F1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00174-3 is reactive to POU5F1 in Human, Mouse, Rat

Applications

A00174-3 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39-45 kDa

Calculated molecular weight

Background of POU5F1

Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. It is mapped to 6p21.33. This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Oct4/POU5F1 using anti-Oct4/POU5F1 antibody (A00174-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oct4/POU5F1 antigen affinity purified polyclonal antibody (Catalog # A00174-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oct4/POU5F1 at approximately 39-45 kDa. The expected band size for Oct4/POU5F1 is at 39 kDa.

Gene/Protein Information For POU5F1 (Source: Uniprot.org, NCBI)

Alternative Names

class 5, transcription factor 1; MGC22487; Oct-3; OCT3Oct4; Oct-4; Octamer-binding protein 3; otc-4; OTF3POU domain class 5, transcription factor 1; OTF4; POU class 5 homeobox 1; POU-type homeodomain-containing DNA-binding protein POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 POU class 5 homeobox 1 POU domain, class 5, transcription factor 1|POU domain transcription factor OCT4|POU-type homeodomain-containing DNA-binding protein|octamer-binding protein 3|octamer-binding protein 4|octamer-binding transcription factor 3

For more info on POU5F1, check out the POU5F1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POU5F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the POU5F1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis