POU5F1 Antibodies

POU domain, class 5, transcription factor 1 (POU5F1)

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Types a trimeric complex with SOX2 on DNA and controls the expression of a variety of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206.

Critical for early embryogenesis and for embryonic stem cell pluripotency. .

Gene Information

Human
Gene Name:	POU5F1
Uniprot:	Q01860
Entrez:	5460

Family

Belongs to:
POU transcription factor family

Alternative Names

class 5, transcription factor 1; MGC22487; Oct-3; OCT3Oct4; Oct-4; Octamer-binding protein 3; otc-4; OTF3POU domain class 5, transcription factor 1; OTF4; POU class 5 homeobox 1; POU-type homeodomain-containing DNA-binding protein

Molecular Weight

Mass (kDA):
38.571 kDA

Genomic Context

Human
Location:	6p21.33
Sequence:	6; NC_000006.12 (31164337..31170682, complement)

Tissue Specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Subcellular Localizations

Cytoplasm. Nucleus. Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Teratoma
• Idiopathic Pneumonia Syndrome
• Embryonal Carcinoma
• Malignant Paraganglionic Neoplasm
• Cell Transformation, Neoplastic
• Testicular Neoplasms
• Germ Cell Tumor
• Leukemia
• Neoplasm Metastasis

• Seminoma
• Neoplasms, Germ Cell And Embryonal
• Chimera Disorder
• Adenocarcinoma
• Idiopathic Hypereosinophilic Syndrome
• Carcinoma In Situ
• Immunologic Deficiency Syndromes

Interacting Proteins

• CTCF
• FOXD3
• Hmgb1
• MBD3
• SOX2

• WWP2

Pathways

• Methylation
• Cell Differentiation
• Dna Methylation
• Cell Proliferation
• Cell Cycle
• Localization
• Gastrulation
• Rna Interference
• Demethylation
• Translation
• Stem Cell Differentiation
• Regeneration
• Cell Development

• Spermatogenesis
• Blastocyst Formation
• Embryo Development
• Pathogenesis
• Cell Death
• Germ Cell Development
• Fertilization

PTMs

• Methylation
• Phosphorylation
• Demethylation
• Acetylation
• Cleavage
• Deacetylation

• Reduction
• Sumoylation
• Ubiquitination
• Dephosphorylation
• Oxidation
• Hydroxylation

• Glycosylation

Research Areas

• Cellular Markers
• Chromatin Research
• Glia Markers
• Neuroscience
• Oligodendrocyte Cell Markers

• Pancreatic Stem Cell Markers
• Stem Cell Markers
• Transcription Factors and Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POU5F1

Best Uses Of The Anti-Oct4/POU5F1 Marker In Boster Bio

Boster Bio is the only company that can detect the Anti Oct4/POU5F1 protein molecule. Boster's antibodies are validated on multiple platforms, with positive and negative samples to assure high affinity and specificity. In addition, Boster rewards first reviewers with product credits and offers their products to scientists world wide. This helps scientists get the most out their research.

Anti-Oct4/POU5F1 marker

Boster Bio's Anti Oct4/POU 5F1 marker has been tested to determine its ability for this gene to be recognized. It is available in two formats - monoclonal and polyclonal - and reacts with the protein in animal samples. Boster Bio developed its POU5F1 antibodies using rabbit and mouse models. This gene plays a critical part in embryogenesis and pluripotency of embryonic cells. It is also known as an associate with MAPK8/MAPK9 in the nucleus. This means that it is essential for stem cell growth.

Oct4 is a crucial factor for lineage priming. It binds with promoters of its genes and regulates the expression of hCG genes. In hES cells Oct4 downregulation coincides with an increase in transcription of the trophoblast genes. Oct4 works with Sox2 to maintain pluripotency.

Oct4 plays a vital role in the early stages embryogenesis. It helps maintain the differentiation potential of progenitors. Later stages of embryogenesis, however, somatic cells may revert back to a naive stage and become the disease-cell type. Single-cell DNA sequencing and genetic lineage tracking have also shown that the Oct4 gene is induced in part by nuclear factor KB. It has been also shown to play a role in the calcification human and mouse valvular vales.

Validation

Pou5f1's gene profil reveals that its enhancer is largely conserved. The chosen reference genes are known to be able to bind to the Pou5f1 enhancer during active transcription in undifferentiated pluripotent mouse ESCs. We performed mock ChIP with a control antibody to confirm this. The enrichment of enhancers sequences was not significant when using the control antibody.

A new factor was used to normalize the expression of Pou5f1 using the geometric averages from three selected genes. The profile of the POU5F1 gene expression was similar to that of its orthologue genes. These genes are found in the human, bovine, and porcine genomes. The gene is present at all developmental stages and expressed at different signal intensities. This includes the oocyte-stage stage. The new normalization factor was used to distinguish between development stages in rabbit embryos from oocytes.

This marker is also believed to inhibit IGFIR activity, a key mediator involved in malignant transformation. IGFIR regulates cell growth, angiogenesis, and metastasis. POU5F1, also known to be octamerbinding transcript factor 4 (Oct-4), controls cell-cycle regulation. This gene controls the self-renewal and regeneration of somatic stem cells. It also maintains lung adenocarcinoma stem cells' stemness.

Applications

The POU5F1 genes encode an octamerbinding transcript factor-4 (OCT4). OCT4 is key to the regulation of pluripotency within embryonic stem cells. This gene is used for identification of these cells and to determine their functional roles. The POU5F1 protein has four isoforms and three transcripts, making it a highly versatile marker.

Homologs for the POU5F1 genetic variant can be found in birds (Xenopus), axolotl and zebrafish. This gene is expressed in both zebrafishes and mice. Humans are homologous to the POU5F1 genetic gene, Pou5f1/Oct4.

TIMER was used to analyze expression profiles from the TCGA. POU5F1 is upregulated in a variety of cancers. These included breast invasive, bladder urothelial, colon, colon, adenocarcinoma as well as head and neck carcinomas. POU5F1 was also associated with B cells.

Nanog, an epigenetic moderator in mouse, is found to regulate Pou5f1 expression in oocytes. Pou5f1 also appears in spermatocytes as well as oocytes from zebrafish, medaka and zebrafish. Pou5f1 is found in all germ cell types, including oocytes, and the large yellow croaker.

Regulatory region

The regulatory region for the POU5F1. The POU5F1 gene can be found in blastocyst embryos. It is expressed in the trophoblast cells lineage. POU5F1 expression decreases after the eighth-to-16th-cell stage in both human and mouse ESCs. During the preimplantation stage of the rabbit embryo, the gene is expressed in both trophoblast and ICM.

For studies of pluripotent stem cell development, the Regulatory region of POU5F gene is particularly interesting. The CR4 domain is responsible to the expression POU5F1in ESCs. Comparative analysis has shown three highly consensus locations in other mammals. In fact, the sequences of CR4 in rabbit are identical to those in human POU5F1 - a strong indication of the conserved regulatory role of this region.

Regulatory region of the POU5F-1 gene is conserved among species and contains two essential elements. These elements are located within a minimal promoter. These elements are essential for the cell-specific regulation of POU5F1 expression. The Sp1/Sp3- and hormone responsive element bindings sites are contained in the minimal promor. Two essential elements were also identified in reporter gene expression experiments that regulate the expression POU5F1 from human ESCs.

RA-induced downregulation

A rabbit POU5F1 indicator was created specifically to detect the downregulation of this particular gene. The POU5F1 promor is highly conserved within mammalian organisms. This regulatory region shares high homology to human POU5F1 genetic material. This marker can thus be used to screen the effect of RA on mouse ESCs.

There weren't any reliable biomarkers available for RA-induced cell death. But Boster Bio has developed a POU5F1/POUV5F1 marker that allows you to identify and analyse RA-induced cell death. The markers are a step toward identifying a new treatment for RA. These markers are useful for doctors to monitor the effectiveness their treatments.

Multiple sequence comparison was used to verify the rabbit POU5F1 sequence. It contains five highly conserved regions that correspond to the POU5F1 gene sequence. This sequence was compared with the cDNA sequence of the reference sequence. The expressed and coding regions were 1083 bp. POU5F1 is now recognized in human, mouse, as well as bovine tissue.

BAC clone

The POU5F1 marker can be used to identify a BAC clone by using a rabbit MHC area. This marker is highly conserved and has five exons as well as a longer 3’ UTR. This gene encodes a hypothetical protein that is almost identical in structure to those of other species and mammals. This indicates that it may play a crucial role in early vertebrate developmental stages.

Multiple sequence alignments of POU5F1 gene, pseudogene and gene have revealed 99% identity. The pseudogene has a frameshift caused by an eight-nucleotide delete. This deletion results to a 162-amino Acid protein lacking the functional POU domain, but maintaining a 92% amino acid level. This sequence is commonly known as EU191070.

A rabbit BAC clone containing a copy of the POU5F1 genes contains a sequence from a conserved section that is very similar other mammalian regulatory parts. The rabbit sequence has the highest homology with a human ortholog and four other mammalian regulatory regions. This result indicates that the POU5F1 marker is a functional marker.

Expression data

The POU5F1 cell-specific transcription factor is controlled through cis-regulatory factors. The POU5F1 promoter carries four conserved areas, the first being a TATA less minimal promoter. The minimal promoter contains two essential elements: the Sp1/Sp3 binding site and the hormone responsive element. Both elements are vital for cell-specific regulation and control of POU5F1.

The POU5F1 genetic code encodes Octamer-binding transcript factor-4 (Oct4). This regulates pluripotency in embryonic stem cells. This gene was studied to identify its promoter region, and examine its expression pattern in rabbit embryos during the preimplantation stage. However, its function remains to be elucidated. It is currently considered a useful molecular marker to embryonic stem cells.

To determine the expression pattern of POU5F1, the POU5F1 promoter region was sequenced and subcloned. It was homologous with four other mammalian regulatory areas. It also showed the highest homology to human genes. This suggests that the POU5F1 marker may be functionally relevant in mammalian developmental.